994 publications from this institution
Recently, a fourth member of the bombesin (Bn) receptor family (fBB4-R) was isolated from a cDNA library from the brain of the frog, Bombina orientalis. Its pharmacology and cell biology are largely unknown, and no known natural cell lines or tissues possess sufficient numbers of fBB4-R's to allow either of these to be determined. To address these issues, we have used three different strategies. fBB4-R expression in cells widely used for other Bn receptor subtypes was unsuccessful as was expression in two frog cell lines. However, stable fBB4-R cell lines were obtained in CHO-K1 cells which were shown to faithfully demonstrate the correct pharmacology of the related Bn receptor, the GRP receptor, when expressed in these cells. [DPhe6,βAla11,Phe13,Nle14]Bn(6−14) was found to have high affinity (Ki = 0.4 nM) for the fBB4 receptor and 125I-[DTyr6,βala11,Phe13,Nle14]Bn(6−14) to be an excellent ligand for this receptor. The fBB4-R had a unique pharmacology for naturally occurring Bn-related agonists, with the presence of a penultimate phenylalanine being critical for high-affinity interaction. It also had a unique profile for six classes of Bn antagonists. The fBB4-R was coupled to phospholipase C with activation increasing [3H]inositol phosphates and mobilizing Ca2+ almost entirely from cellular sources. There was a close correlation between agonist the receptor occupation and the receptor activation. Three of the five classes of Bn receptor antagonists that interacted with higher affinity with the fBB4-R functioned as fBB4-R antagonists and two as partial agonists. fBB4-R activation stimulated increases in phospholipase D (PLD) over the same range of concentrations at which it activated phospholipase C. These results demonstrate that the fBB4 receptor has a unique pharmacology for agonists and antagonists and is coupled to phospholipase C and D. The availability of these cell lines, this novel ligand, and the identification of three classes of antagonists that can be used as lead compounds should facilitate the further investigation of the pharmacology and cell biology of the BB4 receptor.
Despite many recent technical breakthroughs in health care, human behaviour remains the largest source of variance in health-related outcomes (Schroeder, 2007). People’s health and well-being are robustly affected by lifestyle factors such as smoking, hygiene, diet, and physical activity, all of which involve behaviours that are potentially controllable by the individual. In addition, outside of acute care settings, the effectiveness of most health care interventions is highly dependent on the patient’s adherence to self-care activities such as taking medications, performing self-examinations, or refraining from specific activities or habits. A significant problem is the poor adherence to prescribed changes or recommended behaviours over time.
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Organismic theories and recent research suggest that environments that do not support growth and self-expression are associated with valuing financial success relatively more than affiliation, community feeling, and self-acceptance. This prediction was investigated in a heterogenous sample of 18-year-olds using a variety of methods and informants. Teenagers who rated the importance of financial success aspirations relatively high compared to other values were found to have mothers who were less nurturant. Further, materially oriented teenagers grew up in less advantageous socioeconomic circumstances and were raised by mothers who especially valued the teens' financial success. Discussion focuses on explicating the different ways values are acquired.
Questions concerning the nature of happiness and wellness are age-old, as are the ways in which people strive to achieve them. Two traditions dominate theorizing about pathways to happiness and the good life. The hedonic tradition focuses on happiness as a desired subjective outcome, with interest in variables predicting it. The eudaimonic tradition focuses on characteristics associated with living well, defined in terms of realization of human potentials, and views happiness as one by-product of such living. Research within Self-Determination Theory (SDT) has links with each of these traditions. First, SDT explicitly distinguishes wellness from happiness, seeing the latter as a symptom of the former. Second, SDT research provides insights concerning how awareness, self-regulation, and a focus on intrinsic values, all attributes associated with eudaimonia, are associated with both wellness and positive hedonic outcomes, whereas some seemingly hedonistic lifestyles such as materialism can fail to yield even hedonic rewards. Most importantly, SDT highlights how social and environmental factors that support the satisfaction of basic psychological needs for autonomy, competence, and relatedness facilitate happiness and wellness, providing practical, evidence-supported directions for human betterment.
Mindfulness is an attribute of consciousness long believed to promote well-being. This research provides a theoretical and empirical examination of the role of mindfulness in psychological well-being. The development and psychometric properties of the dispositional Mindful Attention Awareness Scale (MAAS) are described. Correlational, quasi-experimental, and laboratory studies then show that the MAAS measures a unique quality of consciousness that is related to a variety of well-being constructs, that differentiates mindfulness practitioners from others, and that is associated with enhanced self-awareness. An experience-sampling study shows that both dispositional and state mindfulness predict self-regulated behavior and positive emotional states. Finally, a clinical intervention study with cancer patients demonstrates that increases in mindfulness over time relate to declines in mood disturbance and stress.
