3,134 publications from this institution
Endoscopic mucosal dissection (ESD) is used to diagnose and treat early gastrointestinal tumors. ESD allows for curative resection of superficial gastrointestinal lesions, with the advantage of treating multiple lesions in a single session and performing repeated procedures when necessary. However, ESD operations require a large field of view, and when bleeding occurs, the restricted space may make it difficult to locate and control the bleeding site in a timely manner. Larger wounds created during ESD procedures require even more space, and while tunneling technology has emerged, it is still considered less direct compared to having an additional hand to open the peeled mucosal window. Although transparent caps can help expose the surgical field, their effectiveness is limited when dealing with larger wounds. Various auxiliary techniques have been explored to address these challenges. In our endoscopic diagnosis and treatment center, we have been using ESD for many years, performing approximately 200 cases annually of gastric mucosal lesions and submucosal masses. The vertical and horizontal margins were negative, and the resection was complete, avoiding surgical treatment. Among these cases, 10 involved the use of dental floss-assisted traction during ESD. Dental floss assistance, as one of the auxiliary ESD methods, has the advantages of convenience and ease of use, which facilitates the imaging process. By securing the tail of a titanium clip with dental floss, the procedure becomes more efficient and adaptable. The traction wire can be pulled as needed during the operation, helping to expose and control the field of view. This significantly supplements the effect of the transparent cap, which may be less effective in larger wound areas. Dental floss traction acts as a third hand, expanding the operational space, facilitating endoscopic maneuvers, reducing surgical time, and minimizing the risk of side injuries during the treatment.
1 póster presentado en el 8th International SedNet Conference "Innovative Sediment Management: How to do more with less", 6-9 November 2013, Lisbon
Single-cell sequencing has gained popularity in recent years. Despite its numerous applications, single-cell DNA sequencing data is highly error-prone due to technical biases arising from uneven sequencing coverage, allelic dropout, and amplification error. With these artifacts, the identification of somatic genomic variants becomes a challenging task, and over the years, several methods have been developed explicitly for this type of data. Single-cell variant callers implement distinct strategies, make different use of the data, and typically result in many discordant calls when applied to real data. Here, we review current approaches for single-cell variant calling, emphasizing single nucleotide variants. We highlight their potential benefits and shortcomings to help users choose a suitable tool for their data at hand.
A phylogenetic model selection test to quantify the evidence for the Universal Common Ancestry (UCA) of life forms was proposed recently (Theobald 2010a), based on the comparison of the statistical support, using likelihoods, the Akaike Information Criterion (AIC), or Bayes factors, for two different phylogenetic models representing the UCA and the independent origins (IOs) hypotheses (Sober and Steel 2002). In this test, the former is represented by a single phylogeny connecting all sequences, whereas the latter is depicted by several, independent phylogenetic trees (Fig. 1). Importantly, in the original UCA test, the same alignment was used to represent both hypotheses. When applied to a particular data set of 23 universally conserved proteins, the test strongly favored a UCA scenario.
Quantitative genetics is the study of continuously varying traits which make up the majority of biological attributes of evolutionary and commercial interest. This book provides a much-needed up-to-date, in-depth yet accessible text for the field. In lucid language, the author guides readers through the main concepts of population and quantitative genetics and their applications. It is written to be approachable to even those without a strong mathematical background, including applied examples, a glossary of key terms, and problems and solutions to support students in grasping important theoretical developments and their relevance to real-world biology. An engaging, must-have textbook for advanced undergraduate and postgraduate students. Given its applied focus, it also equips researchers in genetics, genomics, evolutionary biology, animal and plant breeding, and conservation genetics with the understanding and tools for genetic improvement, comprehension of the genetic basis of human diseases, and conservation of biological resources.
