Publications

To investigate the chemical constituents of Clematis aethusifolia,a traditional Mongolian medicine for resolving hard lump.Bioactive guided isolation and purification of Clematis aethusifolia was performed by normal and reverse phase column chromatography, Sephadex LH-20 and preparation HPLC. The structures were identified by 1D,2D-NMR and MS spectral analysis and comparison with literature data. Cytotoxicity of compounds were determined by CCK-8 cell staining method for detecting growth inhibition to five kinds of human solid tumor cell lines.Eight compounds were isolated and identified as vanillicacid( 1),protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4’-O-β-D-glucopyranoside( 4),( +)-syringaresinol-4’-O-β-Dglucopyranoside( 5),7,9,9’-trihydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside( 6),butyl-β-D-fructopyranoside( 7),butyl-β-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity.All the compounds are isolated from this plant for the first time, Results: Eight compounds were isolated and identified as vanillicacid( 1), protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4’-O-β-D-glucopyranoside( 4),( +)-syringaresinol-4’-O-β-Dglucopyranoside( 5),7,9,9’-trihydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside( 6),butyl-β-D-fructopyranoside( 7),butyl-β-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity. compounds 3,6 ~ 8 are isolated from Clematis genus for the first time, compounds 6 and 8 are isolated from Ranunculaceae family for the first time. Compounds 2,8 may be the effective components in Clematis aethusifolia.

Improving iron utilization efficiency in crops is of great importance to food security and sustainable agricultural development. Quinoa (Chenopodium quinoa

Spot blotch (SB) is a fungal disease that threatens wheat yield and quality. Presently, the molecular mechanism against SB is unclear. In this study, the resistant variety Zhenkang iron shell wheat (Yunmai 0030) and susceptible variety Lincang iron shell wheat (Yunmai 0608) were selected by identifying SB of Yunnan iron shell wheat. The metabolome and transcriptome of leaves of two varieties at different positions were detected using the systemic acquired resistance theory to investigate the molecular and physiological changes in Yunnan iron shell wheat under SB stress. We found that the genes and metabolites related to benzoxazinoid biosynthesis and arginine and proline metabolism were highly enriched after infection with leaf blight. The enriched differential metabolites mainly included phenolic acids, alkaloids, and flavonoids. We further observed that DIBOA- and DIMBOA-glucoside positively affected iron shell wheat resistance to leaf blight and proline and its derivatives were important for plant self-defense. Furthermore, we confirmed that the related metabolites in benzoxazinoid biosynthesis and arginine and proline metabolism positively affected Triticum aestivum ssp. resistance to SB. This study provides new insights into the dynamic physiological changes of wheat in response to SB, helps us better understand the mechanism of resistance to SB, and contributes to the breeding and utilization of resistant varieties.

Aim of the study Rhubarb is well used to treat chronic renal failure (CRF) in China and Japan, but recent studies reported that the anthraquinone derivatives contained in rhubarb had nephrotoxicity. In this investigation an attempt was made to assess the value and toxic potential of rhubarb to treat CRF. Materials and methods Histopathologic and biochemical tests combined with toxicokinetic analysis were performed to investigate the nephrotoxic potential and protective effect of rhubarb extract. Results In normal rat groups, no death was observed and no renal lesion was found after repetitive administration of rhubarb for 3 weeks. The survival rate, pathologic conditions and biochemical indexes of CRF rats treated with rhubarb at two dosages were all improved and significant amelioration was found in the low dosage group compared to the untreated CRF group. Rhein was the mainly absorbable anthraquinone derivative into systemic circulation after oral administration and the area under curve of rhein in CRF groups was lower than that in normal groups at same dosage. Conclusions After 3 weeks of administration of rhubarb extract, there was evidence of protective effect to CRF rats, while incidences of hepatotoxicity with minimal to mild hyaline droplets were also observed in normal rats.

Hedysarum scoparium and Caragana korshinskii seedlings were used as the test materials to carry out the roots exposure stress model test.By comparing the physiological growth indexes in the leaves of the tested plants, the stress resistance ability was evaluated.The results showed (1) root exposure resulted in excessive oxygen free radicals, which resulted in membrane lipid peroxidation and increased MDA content in two plants.The increase of MDA content in H. scoparium was greater than that in C. korshinskii.(2) H. scoparium mainly regulate their own adaptation to the stress by regulating carotenoids.C. korshinskii adjusts itself to adapt to the stress by regulating chlorophyll.(3) H. scoparium resist this stress mainly by regulating their respiration rate.(4) H. scoparium mainly through the mobilization of proline, by adjusting the proline concentration to reduce their water potential; C. korshinskii reduced its water potential mainly by regulating the concentration of soluble sugar to adapt to the stress.(5) H. scoparium and C. korshinskii activated peroxidase (H.scoparium) and catalase (C.korshinskii) to clean up intracellular peroxides, respectively.To sum up, under the same root exposure ratio, there were significant differences in physiological regulation and morphological indexes between H. and C. korshinskii, but their stress resistance mechanisms were quite different.

Lilium lancifolium is a plant resource used as both medicine and food because it is enriched with polysaccharides, polyphenol compounds, and saponins. Increasing the quality of Lilium species is based largely on improvement using methods such as selective breeding and proper fertilization. In this study, we investigated the different responses of L. lancifolium bulbs to treatment with nitrogen (N) and potassium (K) in Hoagland solution. A pot experiment was conducted with four N rates and five K rates under a completely random design. The agronomic traits, N and K contents, and concentrations of active compounds were determined in bulbs, including total phenols, flavonoids, polysaccharides, and saponins. L. lancifolium treated with N and K exhibited increases in the plant height, leaf number, and chlorophyll content compared with the control (N0 + K0). The bulb circumference increased by 17.41% under N2 (609.80 mg L−1) + K2 (523.34 mg L−1) compared with N0 + K0. Individual or combined application of N and K increased the total phenol, flavonoid, and saponin contents, especially under N2 + K3, with the highest increases of 1.87–2.93 times compared with N0 + K0. However, the individual application of N decreased the polysaccharide contents by 2.78–42.04%. Hoagland solution containing 443.24–572.87 mg L−1 N and 573.61–759.16 mg L−1 K is recommended to improve the active contents of bulb components based on regression analysis. Our results demonstrate that the combined application of N and K is important for obtaining high-quality L. lancifolium bulbs.

Our previous work has indicated that salicylic acid (SA) is involved in the development of pea plant thermotolerance and induces the gene expression of phe

Background： By establishing the tissue rapid propagation system, the rapid propagation and large yield were not affected by external factors, and the scarcity of wild resources was effectively solved. Analysis of test-tube plantlets and wild plantlets by ISSR molecular marker technology to detect the stability of genetic characteristics of test-tube plantlets is of great significance to the study of germplasm resources and medicinal value of Leptospiraea japonica. Result ： The regeneration system of Rubus sachalinensis Leveille was established in this paper by using axillary buds as explants in vitro culture. The results showed that axillary buds of Rubus sachalinensis Leveille were sterilized in 75% alcohol for 40 s and then in 0.1% HgCl 2 for 8 min had the best effect, the pollution and the browning rate of the explants reached a rather low level, with the survival rate of 78.34%. The optimum compositions for proliferation culture, strong plant culture and root inducing were MS + 6-BA 2.0 mgL -1 + KT 0.5 mgL -1 + NAA 0.1 mgL -1 + IBA 0.05 mgL -1 , MS + 6-BA 2.0 mgL -1 + KT 0.5 mgL -1 + NAA 0.1 mgL -1 + IBA 0.1 mgL -1 + GA 3 0.5 mgL -1 and 1/2 MS + IBA 1.5 mgL -1 . The proliferation coefficient and rooting rate on these media were up to 7.71, 6.60, and 97.04% respectively. The most suitable transplanting medium for test-tube plantlets was a mixed medium of humus, vermiculite and perlite ( 2 : 1 : 1 v / v ), and the survival rate of transplanting was 100 %. This study was conducted to acquire a large quantity of wide Rubus sachalinensis Leveille plantlets and provide a basis for large-scale production.The tissue culture seedlings were amplified by PCR with simple repeat interval. Conclusion ：The results showed that the tissue culture seedlings had high genetic stability and low mutation rate compared with wild seedlings.This study not only opens up a new way for the tissue rapid propagation technology of L. japonica, but also lays a foundation for the maintenance of excellent traits and resource protection of L. japonica.

Secondary lymphoid tissue chemokine (SLC/CCL21), one of the CC chemokines, exerts potent antitumor immunity by co‐localizing T cells and dendritic cells at the tumor site and is currently tested against human solid tumors. Here, we investigated whether the combination of recombinant adenovirus encoding murine CCL21 (Ad‐ mCCL 21) with low‐dose paclitaxel would improve therapeutic efficacy against murine cancer. Immunocompetent mice bearing B16‐F10 melanoma or 4T1 breast carcinoma were treated with either Ad‐ mCCL 21, paclitaxel, or both agents together. Our results showed that Ad‐ mCCL 21 + low‐dose paclitaxel more effectively reduced the growth of tumors as compared with either treatment alone and significantly prolonged survival time of the tumor‐bearing animals. These antitumor effects of the combined therapy were linked to altered cytokine network at the tumor site, enhanced apoptosis of tumor cells, and decreased formation of new vessels in tumors. Importantly, the combined therapy elicited a strong therapeutic antitumor immunity, which could be partly abrogated by the depletion of CD4 + or CD8 + T lymphocytes. Collectively, these preclinical evaluations may provide a combined strategy for antitumor immunity and should be considered for testing in clinical trials.