Publications

蒙薬細叶蒙薬細叶の化学成分の研究【JST・京大機械翻訳】

No abstract is provided for this article.

Joint analysis of transcriptome and metabolome on the accumulation mechanism of flavonoids in quinoa seedlings under flooding stress

Physiological and molecular bases of the boron deficiency response in tomatoes

Boron is an essential microelement for plant growth. Tomato is one of the most cultivated fruits and vegetables in the world, and boron deficiency severely inhibits its yield and quality. However, the mechanism of tomato in response to boron deficiency remains largely unclear. Here, we investigated the physiological and molecular bases of the boron deficiency response in hydroponically grown tomato seedlings. Boron deficiency repressed the expression of genes associated with nitrogen metabolism, while it induced the expression of genes related to the pentose phosphate pathway, thereby altering carbon flow to provide energy for plants to cope with stress. Boron deficiency increased the accumulation of copper, manganese and iron, thereby maintaining chlorophyll content and photosynthetic efficiency at the early stage of stress. In addition, boron deficiency downregulated the expression of genes involved in cell wall organization and reduced the contents of pectin and cellulose in roots, ultimately retarding root growth. Furthermore, boron deficiency markedly altered phytohormone levels and signaling pathways in roots. The contents of jasmonic acid, jasmonoy1-L-isoleucine, trans-zeatin riboside, abscisic acid, salicylic acid, and SA glucoside were decreased; in contrast, the contents of isopentenyladenine riboside and ethylene precursor 1-aminocyclopropane-1-carboxylic acid were increased in the roots of boron-deficient tomato plants. These results collectively indicate that tomato roots reprogram carbon/nitrogen metabolism, alter cell wall components and modulate phytohormone pathways to survive boron deficiency. This study provides a theoretical basis for further elucidating the adaptive mechanism of tomato in response to boron deficiency.

Retraction: MZF1 alleviates oxidative stress and apoptosis induced by rotenone in SH-SY5Y cells by promoting RBM3 transcription

Editor's AnnouncementMZF1 alleviates oxidative stress and apoptosis induced by rotenone in SH-SY5Y cells by promoting RBM3 transcriptionJi Zhang, Wen Chai, Zhengbing Xiang, Xinhua Zhou, Ping Zhang(The Journal of Toxicological Sciences, 46, 477-486, 2021)This paper has been withdrawn at the request of the authors.This article was accepted for publication by the Editorial Committee of The Journal of Toxicological Sciences after a regular peer review process. However, the Editorial Committee received an email from the corresponding author, Dr. Ping Zhang, requesting that the paper be withdrawn. The Editorial Board asked him to submit a letter of consent from all co-authors, and he subsequently provided one with the signatures of all co-authors. As that letter attested to the authors' intention, we approved the withdrawal of this paper.The authors have not disclosed the reasons for the withdrawal. However, the Editorial Committee has decided to respect their decision. Therefore, this action was taken at the authors' own responsibility.Toshiyuki Kaji, Ph.D. Editor-in-ChiefThe Journal of Toxicological Sciences.

NtPIN3 positively regulates low phosphorus tolerance by changing root elongation, Pi concentration and antioxidant capacity in tobacco

Phosphorus (P) is a critical nutrient for plants, and inorganic orthophosphate (Pi) deficiency results in declining crop quality and yields. Auxin plays a key regulatory effect on plant growth in response to Pi deficiency, yet the regulatory mechanisms remain poorly understood. In this study, we cloned and identified an auxin efflux transporter, NtPIN3, involved in the low phosphate response of tobacco (Nicotiana tabacum). NtPIN3 is located on the cytoplasmic membrane, and high expression levels of NtPIN3 were observed under phosphorus deficiency conditions. Moreover, NtPIN3 overexpression in tobacco could dramatically enhance low Pi tolerance by increasing the root length, which was consistent with the GUS activity and the auxin concentration in the root. Notably, Pi concentration and antioxidant capacity in overexpression transgenic tobacco were significantly increased compared with the wild type (WT) under low Pi stress conditions due to Pi uptake (NtPT1 and NtPT2) and antioxidant capacity (superoxide dismutase, peroxidase, and catalase). In contrast, compared with WT, the ntpin3 mutant significantly reduced root length, auxin concentration, Pi concentration, and antioxidant capacity under low Pi stress conditions, suggesting that NtPIN3 is a positive regulator of low phosphate in tobacco. In addition, the yeast-two-hybrid and luciferase complementation imaging assay confirmed that NtPIN3 interacted with NtPIN2. Altogether, the NtPIN3 involved in low Pi response and overexpression of NtPIN3 contributed to enhanced tolerance to low Pi stress, which proved an underlying gene for breeding low Pi-stress tolerant plants.

Problems on experimental design and result analysis in manuscripts submitted to Acta Horticulturae Sinica.

Ferula kryloviiとPeganum harmalaにおける呼吸とフォースインテシスに及ぼす温度の影響【Powered by NICT】

No abstract is provided for this article.

A magnetic γ-Fe₂O₃@PANI@TiO₂ core–shell nanocomposite for arsenic removal <i>via</i> a coupled visible-light-induced photocatalytic oxidation–adsorption process

Arsenic polluted groundwater impairs human health and poses severe threats to drinking water supplies and ecosystems. Hence, an efficient method of simultaneous oxidation of As(iii) to As(v), and removal of As(v) from water has triggered increasing attention. In this study, a magnetic γ-Fe

Peer Review #2 of "Comparative analysis of physiological responses to environmental stress in Hedysarum scoparium and Caragana korshinskii seedlings due to roots exposure (v0.3)"

Hedysarum scoparium and Caragana korshinskii seedlings were used as the test materials to carry out the roots exposure stress model test.By comparing the physiological growth indexes in the leaves of the tested plants, the stress resistance ability was evaluated.The results showed (1) root exposure resulted in excessive oxygen free radicals, which resulted in membrane lipid peroxidation and increased MDA content in two plants.The increase of MDA content in H. scoparium was greater than that in C. korshinskii.(2) H. scoparium mainly regulate their own adaptation to the stress by regulating carotenoids.C. korshinskii adjusts itself to adapt to the stress by regulating chlorophyll.(3) H. scoparium resist this stress mainly by regulating their respiration rate.(4) H. scoparium mainly through the mobilization of proline, by adjusting the proline concentration to reduce their water potential; C. korshinskii reduced its water potential mainly by regulating the concentration of soluble sugar to adapt to the stress.(5) H. scoparium and C. korshinskii activated peroxidase (H.scoparium) and catalase (C.korshinskii) to clean up intracellular peroxides, respectively.To sum up, under the same root exposure ratio, there were significant differences in physiological regulation and morphological indexes between H. and C. korshinskii, but their stress resistance mechanisms were quite different.

Cultivating the Spirit of Aesthetic Education in Vocational Undergraduate Colleges in the Context of Artificial Intelligence

The fusion of aesthetic education with vocational training constitutes a necessary evolution dictated by both societal progression and the demands of contemporary times, with each element enhancing the other. This study proposes a pathway for the construction of aesthetic education courses tailored to the current educational objectives of vocational undergraduate institutions. It also introduces a teaching model designed to foster aesthetic sensibilities structured around the ADDIE instructional design framework. Moreover, this research employs the 2023 student cohort from HZ Vocational College as subjects for a comparative experimental study to empirically analyze the effectiveness of the aesthetic education teaching approach. An enhanced clustering algorithm, which integrates multi-point optimization, was applied to assess the development of students’ aesthetic spirit. Additionally, a regression analysis model was used to investigate the various factors influencing the cultivation of this aesthetic spirit, providing a comprehensive analysis of the impact of the proposed educational interventions. The results show that after the teaching, the mean values of student evaluation, self-evaluation, parent evaluation, and teacher evaluation in the five aspects of recognizing beauty, experiencing beauty, feeling beauty, appreciating beauty, and creating the beauty of the students in Class A of the sample are higher than those of Class B by 4.90, 4.08, 3.40 and 4.13. Their p-values are all statistically significant at 0.000&lt;0.05. The ADDIE-based aesthetic education teaching model can effectively cultivate students’ aesthetic spirit and provide important value references for the reform and development of aesthetic education.

Morphological and Phylogenetic Evidences Reveal Four New Species of Cantharellus Subgenus Cantharellus (Hydnaceae, Cantharellales) From China

Species of Cantharellus subgenus Cantharellus are interesting and important for their mycorrhizal properties, medicinal values, and edibility. In China, there are many undescribed species of the subgenus. In this study, four new species of subg. Cantharellus , viz. Cantharellus albopileatus , Cantharellus chuiweifanii , Cantharellus pinetorus , and Cantharellus ravus from Hainan and Hunan Provinces, respectively, were described based on morphological and phylogenetic evidence as a contribution to the knowledge of the species diversity in China. Detailed descriptions, color photographs of fresh basidiomata, and line drawings of microstructures of these four new species are presented as well as comparisons with related species.

Identification of core genes associated with different phosphorus levels in quinoa seedlings by weighted gene co-expression network analysis

Quinoa is a highly nutritious and novel crop that is resistant to various abiotic stresses. However, its growth and development is restricted due to its limited utilization of soil phosphorus. Studies on the levels of phosphorus in quinoa seedlings are limited; therefore, we analyzed transcriptome data from quinoa seedlings treated with different concentrations of phosphorus.To identify core genes involved in responding to various phosphorus levels, the weighted gene co-expression network analysis method was applied. From the 12,085 expressed genes, an analysis of the gene co-expression network was done. dividing the expressed genes into a total of twenty-five different modules out of which two modules were strongly correlated with phosphorus levels. Subsequently we identified five core genes that correlated strongly either positively or negatively with the phosphorus levels. Gene ontology and assessments of the Kyoto Encyclopedia of Genes and Genomes have uncovered important biological processes and metabolic pathways that are involved in the phosphorus level response.We discovered crucial new core genes that encode proteins from various transcription factor families, such as MYB, WRKY, and ERF, which are crucial for abiotic stress resistance. This new library of candidate genes associated with the phosphorus level responses in quinoa seedlings will help in breeding varieties that are tolerant to phosphorus levels.

Two Methods Detect the Tyrosine Phosphorylation of STAT3

Objective FCM(flow cytometry) is compared with Western blot in investigating the phosphorylated of STAT3.Methods Promyelocytic leukemia cell line HL-60 are cultured and stimulate with human IL(hIL)-5 in different concentration and then are detected with Western Blot and FCM simultaneously.Results The results of Western blot and FCM are identical.Conclusion The two methods are all have their own advantages in investigating phosphorylated STAT3 and they are mutually complementary.

Design and Realization of the Multi-Function Tower CraneBlock Box Based on ZigBee

This paper introduces the design and realization of the Multi-function Tower Crane Black Box, completes the main hardware module design and type selection. With ZigBee, it constructing wireless sensor network to collect the communication among nodes and the communication between nodes and the gateway, the lower PC adopts the IAR to complete the chip CC2430 programming. The application development tool for the upper PC is C51RF - WSN - DA300, and it gives part of the source code. The system parameters acquisition is accurate, the operation is stable, and the actual application effect is good.

[Preparation and characterization of human colon tumor-associated antigen and its clinical significance].

To purify the colon tumor-associated antigen from cultured colon tumor cells, and to investigate its expression in the sera of patients with colon cancer.The monoclonal antibody (mAb) against human colon tumor-associated antigen 4D10 was employed as the ligand for the immunoaffinity chromatography to purify the colon tumor-associated antigen from the lysate of colorectal tumor cell LOVO. The purified antigen was identified by SDS-PAGE and Western blot. The expression of colon tumor-associated antigen in the sera of patients with colon cancer and in normal sera was detected by Sandwich ELISA.The purified colon tumor-associated antigen binding to mAb 4D10 was a heterodimer composed of two subunits with relative molecular mass M(r) of 30 x 10(3) and 35 x 10(3) respectively. The antigen was significantly higher expressed in sera from patients with colon cancer than that in normal sera (P<0.01).The tumor-associated antigen obtained from the colon tumor cells has been successfully purified through immunoaffinity chromatography with mAb 4D10, which may be useful for diagnosis on clinic.

A Dynamic Distance Measurement of Vehicle Safety Steering.

A teosinte-derived allele of ZmSC improves salt tolerance in maize

Maize, a salt-sensitive crop, frequently suffers severe yield losses due to soil salinization. Enhancing salt tolerance in maize is crucial for maintaining yield stability. To address this, we developed an introgression line (IL76) through introgressive hybridization between maize wild relatives Zea perennis, Tripsacum dactyloides, and inbred Zheng58, utilizing the tri-species hybrid MTP as a genetic bridge. Previously, genetic variation analysis identified a polymorphic marker on Zm00001eb244520 (designated as ZmSC), which encodes a vesicle-sorting protein described as a salt-tolerant protein in the NCBI database. To characterize the identified polymorphic marker, we employed gene cloning and homologous cloning techniques. Gene cloning analysis revealed a non-synonymous mutation at the 1847th base of ZmSCIL76 , where a guanine-to-cytosine substitution resulted in the mutation of serine to threonine at the 119th amino acid sequence (using ZmSCZ58 as the reference sequence). Moreover, homologous cloning demonstrated that the variation site derived from Z. perennis. Functional analyses showed that transgenic Arabidopsis lines overexpressing ZmSCZ58 exhibited significant reductions in leaf number, root length, and pod number, alongside suppression of the expression of genes in the SOS and CDPK pathways associated with Ca2+ signaling. Similarly, fission yeast strains expressing ZmSCZ58 displayed inhibited growth. In contrast, the ZmSCIL76 allele from Z. perennis alleviated these negative effects in both Arabidopsis and yeast, with the lines overexpressing ZmSCIL76 exhibiting significantly higher abscisic acid (ABA) content compared to those overexpressing ZmSCZ58 . Our findings suggest that ZmSC negatively regulates salt tolerance in maize by suppressing downstream gene expression associated with Ca2+ signaling in the CDPK and SOS pathways. The ZmSCIL76 allele from Z. perennis, however, can mitigate this negative regulatory effect. These results provide valuable insights and genetic resources for future maize salt tolerance breeding programs.

Inhibition efficiency of natamycin pretreatment before harvest on gray mold of post-harvest grapes.

No abstract is provided for this article.