Bu çalışmada, onbir bitki familyasından yirmi türün Orosanga japonica nimf kontrolüne karşı potansiyel etkinlikleri edilmiştir. Etil Asetat özütleri için, en yüksek mortalite oranlarını Daucus carota ve Salvia vertisillate vermiştir. En düşük mortalite 24 saat sonra Matricaria camomilla ve 48 saat sonra Tripleurospermum caucasicum için bulunmuştur. Metanol ekstraktlarında, en düşük ölüm oranları iki test süresinde de Matricaria camomilla için bulunmuştur. En yüksek mortalite oranları 24 saat Salvia verticillate ve 48 saat için Salvia verticillate, Erigeron annuus, Daucus carota için bulunmuştur. Metanol ekstraktları ölüm oranlarının genellikle etil asetat ekstraktlarından daha yüksek olduğu bulunmuştur. En etkili bitki ham özütleri sırasıyla Salvia verticillate (Lamiaceae) ve Daucus carota (Apiaceae) olarak görülmüştür. Sonuç olarak, on bir farklı bitki familyası türlerinden alınan özütlerin Orosanga nimflerine karşı yüksek veya orta derecede potansiyel insektisidal aktivite rapor edilmiştir. Bu nedenle Karadeniz ülkelerinde istilacı Orosanga japonica'ya karşı biyolojik bazlı kontrol yöntemlerinin oluşturulması için kapsamlı ve daha ayrıntılı bir çalışma yapılması gerekmektedir.
Control of Asian tiger mosquito is very crucial for newly established areas. Botanically based insecticides could be used more as alternative biocontrol agents in the future. The ethyl acetate extracts of some plants naturally growing in Turkey were analysed for potential oviposition deterrent, ovicidal, and skin repellent activities against Aedes albopictus. According to the determined oviposition activity index and per cent effective repellence of these plant extracts at constant concentration in the first week, the most effective result belonged to Salvia verticillata (-0.9942 and 99.7%, respectively). In these trials for four weeks, the best persistence belonged to Matricaria chamomilla. In addition to this test, the ovicidal activities and skin repellent activities for the same plant extracts were determined. Qualitative phytochemical analyses of all plants were performed and showed that alkaloids, flavonoids, terpenoids, coumarins, quinones, tannins, phenols and carbohydrates were present in varying proportions in all plants. All of evidence showed that the plant extracts could be used as potential oviposition deterrents, ovicides and repellents against Aedes albopictus.
An invasive Aedes Albopictus (Skuse, 1894) (Diptera: Culicidae) is considered one of the most crucial disease vectors. In this study, we aimed to monitor gradual changes in insecticide resistance profiles of Ae. Albopictus populations have recently invaded the Turkish territory. We collected Ae. Albopictus specimen from ten localities in the Black Sea, Marmara and Aegean regions in subsequent years. Adult Ae. Albopictus insecticide susceptibility to deltamethrin (0.05%), cyfluthrin (0.15%), etofenprox (0.5%), bendiocarb (0.1%), fenitrothion (1%) and dichloro diphenyl trikloroetan (DDT) (4%) was determined by the susceptibility test of World Health Organisation. Results showed common pyrethroid susceptibility. Bendiocarb and fenitrothion resistance was more common in the study. Interestingly, DDT resistance was still high in some populations despite the prohibition of the insecticide in Türkiye. A comparison of resistance status in two years demonstrated that populations were not under pyrethroid selection pressure in the study area. The fact that the bendiocarb and fenitrothion resistance, which was present when the populations were first established, continues after two years, demonstrates that the populations are under selection pressure in the study area. We believe that the findings in the study would help resistance management which is a major component of Ae. Albopictus control efforts.
Aedes aegypti (L., 1762) ve Aedes albopictus (Skuse, 1895) (Diptera: Culicidae) birçok arbovirüsü taşıyan çok önemli vektörlerdir. Türkiye’de Ae. albopictus yumurtaları ilk defa 2011 yılında Türkiye’nin kuzeybatısında bulunan Trakya’da tespit edilmiştir. 2015 yılında ise yapılan çalışmalarla, Ae. albopictus’un yayılımını genişlettiği ortaya çıkarılmış ve Ae. aegypti türü de Türkiye’nin kuzeydoğusunda ilk defa tespit edilmiştir. Bu çalışma Ae. albopictus ve Ae. aegypti’nin Türkiye’de 5 yıl boyunca yayılım ve dağılımlarına dair bulgular veren ilk çalışmadır. 2019 yılı itibarıyla Ae. albopictus ve Ae. aegypti’nin varlığını tespit etmek için 5 coğrafi bölgede (Karadeniz, İç Anadolu, Marmara, Akdeniz ve Ege Bölgeleri) çalışmalar yapılmıştır. Çalışmada lastikler, yapay konteynerler, mezarlıklar, su şişeleri ve doğal üreme habitatları gibi tüm potansiyel larva üreme alanları kontrol edilmiştir. Ayrıca bazı alanlarda ovitrap ve ergin tuzakları da kullanılarak örneklemeler yapılmıştır. Sonuçlar, Ae. albopictus’un her yıl yayılımını arttırdığını ve gelecek yıllarda tüm Türkiye’de yayılma potansiyeli taşıdığını göstermiştir. Aedes aegypti’nin yayılımı ise şimdilik sadece kuzeydoğu ile sınırlı kalmıştır. Gelecekte yapılabilecek daha efektif sürveyans ve kontrol çalışmaları da tartışılmıştır.
Culex pipiens L., 1758 (Diptera: Culicidae) dünyadaki en önemli ve hastalık vektörü olan türlerden biridir. Efektif bir kontrol yapılabilmesi için insektisitlere karşı gelişen direnci takip etmek büyük öneme sahiptir. Bu çalışmada Türkiye Orta ve Doğu Karadeniz Bölgesi’nde 2020 aktif sezonunda dokuz ilden toplanan Cx. pipiens örneklerinde vgsc (kdr L1014F/C) ve ace-1 (G119S, F290V) spesifik bölgelerinde direnç ile ilgili mutasyonların varlığı araştırılmıştır. kdr için, her bölgede L1014F mutasyonu belirlenirken, yabanıl ve dirençli tip aleller için üç farklı sessiz mutasyon tespit edilirken çalışılan popülasyonların hiçbirinde L1014C mutasyonu saptanmamıştır. ace-1 bölgesi için, çalışılan popülasyonlarda F290V değişimi heterozigot ve düşük oranlarda saptanırken, G119S değişimi daha yaygın bulunmuştur. ace-1 bölgesi için G119I (6 popülasyon) ve G119A (5 popülasyon) değişimleri ilk defa tespit edilmiştir. Dirence neden olan mutasyon tiplerinde yapay ve tarımsal alanlar arasında anlamlı fark bulunamamıştır.
Invasive Aedes mosquitoes are established in certain locations of northeastern Turkey. Herein we conclusively show the role of these species in WNV circulation in the region. Biosurveillance is imperative to monitor the spread of these species further into Asia Minor and to detect possible introduction of pathogens.
Aedes aegypti (L., 1762) (Diptera: Culicidae) is an important pest for human health. It vectors many diseases, including yellow fever (YF), dengue (DENV), and chikungunya (CHIKV). This species has invaded numerous countries including Balkan and Caucasian countries few past decades. The established populations of species were found in Turkey in 2015. We investigated the genetic variation, molecular phylogenetics, and differences between samples from Turkey and Georgia using four mitochondrial and one genomic DNA markers. The possible origin of the species was determined separately for each gene region using maximum likelihood trees. The ML analysis showed a close relation with the Caucasian samples, and some haplotypes are specific to this region. Our results suggest that Turkish Ae. aegypti strains might have been a mix of Asian and American strains. The differences between Turkish and Georgian samples were statistically insignificant and values of difference were very low according to AMOVA. Pairwise difference values between the two countries indicated that populations may have the same origin and variation value between two countries is very low. As a conclusion, our results revealed that our region (Turkey and Georgia) samples were most probably a new invasion rather than an ancient one.
Aedes (Stegomyia) albopictus (Skuse, 1894) is an invasive mosquito species that can transmit many arboviral diseases. In Turkiye, this species was found first in Thrace in 2011, then in the Eastern Black Sea in 2014, and in the Aegean regions in 2018. We analyzed the COI gene region of 554 samples from 13 different locations in the Black Sea, Aegean, and Marmara regions to determine the COI diversity and possible introduction origin of A. albopictus in Türkiye. Three haplotypes derived from two segregation sites were detected, and the Hd and π values were 0.175 and 0.00029, respectively. Hap_1 was a common haplotype and was detected in all regions. Hap_2 was detected in the Black Sea and Marmara regions, while Hap_3 was rare, and recorded only in Aegean lineages. In pairwise FSTs of 13 geographical populations, the Marmara lineages were statistically different from the Black Sea and Aegean lineages (except the Mugla samples). AMOVA test results indicated significant differences between the three studied regions (df = 2, f = 0.01784). Although Turkish Aedes albopictus lineages appear to have originated from temperate Northern Italy lineages and Aegean lineages may have originated from a mix of Italy and Albania lineages, the results revealed multiple introduction events via various routes.
Culex pipiens species complex is commonly found in tropical and subtropical areas. Identification of species complexes and their spatial distribution patterns are fundamental steps needed to improve our understanding of arboviral disease circulation and their control. The specimens were differentiated using the Ace-2 gene, and the ecological forms of Culex pipiens were identified by analysis of the CQ11 microsatellite locus. Results showed that all sample points comprised of Culex pipiens s.s., and seven points contained Culex torrentium. Further, we determined that 22 haplotypes from five haplogroups were identified within the Ace-2 marker, and three haplotypes were contained within the CQ11 microsatellite locus. We determined that there was a high degree of the genetic flow of each marker between populations. In conclusion, Culex pipiens complex and sibling species were determined to be distributed throughout the coastal and inland parts of the Black Sea region in Turkey. The occurrence of two distinct ecological forms has the potential to create hybridization zones and a high risk of WNV transmission.
Accurate identification of insect species is an indispensable and challenging requirement for every entomologist, particularly if the species is involved in disease outbreaks. The European MediLabSecure project designed an identification (ID) exercise available to any willing participant with the aim of assessing and improving knowledge in mosquito taxonomy. The exercise was based on high-definition photomicrographs of mosquitoes (26 adult females and 12 larvae) collected from the western Palaearctic. Sixty-five responses from Europe, North Africa and the Middle East were usable. The study demonstrated that the responders were better at identifying females (82% correct responses) than larvae (63%). When the responders reported that they were sure of the accuracy of their ID, the success rate of ID increased (92% for females and 88% for larvae). The top three tools used for ID were MosKeyTool (72% of responders), the ID key following Becker et al. [2010. Mosquitoes and their control, 2nd edn. Berlin: Springer] (38%), and the CD-ROM of Schaffner et al. [2001. Les moustiques d’Europe: logiciel d’identification et d’enseignement – The mosquitoes of Europe: an identification and training programme. Montpellier: IRD; EID] (32%), while other tools were used by less than 10% of responders. Responders reporting the identification of mosquitoes using the MosKeyTool were significantly better (80% correct responses) than non-MosKeyTool users (69%). Most responders (63%) used more than one ID tool. The feedback from responders in this study was positive, with the exercise being perceived as halfway between educational training and a fun quiz. It raised the importance of further expanding training in mosquito ID for better preparedness of mosquito surveillance and control programmes.
