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Land-use change, especially from forest to intensive agriculture, is negatively impacting soil quality and sustainability. Soil biological activities are sensitive indicators of such land-use impacts. We tested two hypotheses: i) land use and management practices affect microbial properties (microbial biomass and enzyme activities) in topsoil (0–20cm), but have no effects in subsoil (20–100cm); and ii) microbial properties in topsoil are highest in forest, followed by organic farming and then conventional farming. Total organic C and N contents as well as microbial biomass were significantly higher in the organic farming topsoil compared with conventional farming and forest. Except xylanase and acid phosphatase, enzyme activities (β-glucosidase, cellobiohydrolas, chitinase, sulfatase, leucine aminopeptidase and tyrosine aminopeptidase) were also higher in organic farming soil. Crop residues and rhizodeposits support higher microbial biomass, leading to enhanced enzyme activities in organic farming soil. Incorporation of rice stubble and limitation of available phosphorus explain the higher xylanase and acid phosphatase activities, respectively, in conventional farming soil. Litter removal leads to a deficiency of labile C and N, resulting in lower enzyme activities in forest soil. Total C and N contents were higher in subsoil under organic farming. Although there was no effect of land use on microbial biomass in subsoil, activities of most enzymes were higher under organic farming. Overall, our results indicate that land-use change significantly alters microbial properties in topsoil, with modest effects in subsoil. Microbial properties should be considered in environmental risk assessments and models as indicators of ecosystem disturbance caused by land-use and management practices.

Increasing the belowground translocation of assimilated carbon by plants grown under elevated CO 2 can cause a shift in the structure and activity of the microbial community responsible for the turnover of organic matter in soil. We investigated the long‐term effect of elevated CO 2 in the atmosphere on microbial biomass and specific growth rates in root‐free and rhizosphere soil. The experiments were conducted under two free air carbon dioxide enrichment (FACE) systems: in Hohenheim and Braunschweig, as well as in the intensively managed forest mesocosm of the Biosphere 2 Laboratory (B2L) in Oracle, AZ. Specific microbial growth rates ( μ ) were determined using the substrate‐induced respiration response after glucose and/or yeast extract addition to the soil. For B2L and both FACE systems, up to 58% higher μ were observed under elevated vs. ambient CO 2 , depending on site, plant species and N fertilization. The μ‐ values increased linearly with atmospheric CO 2 concentration at all three sites. The effect of elevated CO 2 on rhizosphere microorganisms was plant dependent and increased for: Brassica napus = Triticum aestivum < Beta vulgaris < Populus deltoides . N deficiency affected microbial growth rates directly (N limitation) and indirectly (changing the quantity of fine roots). So, 50% decrease in N fertilization caused the overall increase or decrease of microbial growth rates depending on plant species. The μ‐ value increase was lower for microorganisms growing on yeast extract then for those growing on glucose, i.e. the effect of elevated CO 2 was smoothed on rich vs. simple substrate. So, the r/K strategies ratio can be better revealed by studying growth on simple (glucose) than on rich substrate mixtures (yeast extract). Our results clearly showed that the functional characteristics of the soil microbial community (i.e. specific growth rates) rather than total microbial biomass amount are sensitive to increased atmospheric CO 2 . We conclude that the more abundant available organics released by roots at elevated CO 2 altered the ecological strategy of the soil microbial community specifically a shift to a higher contribution of fast‐growing r‐selected species was observed. These changes in functional structure of the soil microbial community may counterbalance higher C input into the soil under elevated atmospheric CO 2 concentration.

Anthropogenic activities have substantially increased soil nutrient availability, which in turn affects ecosystem processes and functions, especially in nutrient-limited ecosystems such as alpine grasslands. Although considerable efforts have been devoted to understanding the responses of plant productivity and community composition to nitrogen (N) and phosphorus (P) enrichment, the nutrient enrichment effects on soil organic carbon (SOC) and microbial functions are not well understood. A four-year field experiment was established to evaluate the influence of continuous N and P enrichment on plant growth and SOC content in an alpine grassland of the Qinghai-Tibetan Plateau. The study included four treatments: Control without addition, N addition, P addition, and N plus P addition. N addition strongly increased aboveground plant biomass and decreased species richness by promoting growth of the dominant grasses species. In contrast, N and P enrichment significantly decreased SOC, especially the recalcitrant organic C content in the surface layer (0–10 cm) by reducing the slow C pool and enlarging the active C pool. Microbial biomass and activities of C-degrading enzymes (β-glucosidase, cellulase and polyphenol oxidase) and an N-degrading enzyme (chitinase) increased with nutrient inputs. The CO2 emissions during a 300 d incubation period were positively correlated with the cellulase and chitinase activities, while the slow C pool was negatively correlated with the cellulase and polyphenol oxidase activities. Consequently, N and P enrichment accelerated decomposition of the recalcitrant C by stimulating microbial growth and increasing enzyme activities, leading to negative impacts on soil C sequestration. Overall, the results indicate that alpine grassland soils of the Qinghai-Tibetan Plateau may be changing from a C sink to a C source under increasing N and P availability, and improvement of alpine grassland management through nutrient inputs should consider not only the aboveground biomass for grazing, but also the soil C sequestration and ecosystem functioning.

Grasslands store large amounts of C; however, the underlying mechanisms of soil C sequestration after grazing exclusion are not well known. This study aime

Context or Problem Numerous studies have demonstrated that biochar application can increase crop yield by improving soil properties and health. Yet, these studies, however, neglected how biochar alters yield variability across years – reflecting the yield stability. Objective or Research question This study aimed to investigate the effects of biochar application on crop yield variability. Methods Published data from 38 experimental sites were collected from the Web-of-Science. Two-thirds of the data were originated from three main staple crops: maize, wheat, and rice. The remaining were from rapeseed, soybean, sweet potato, and peppermint. Biochar effects on crop yield and its variability as well as their driving factors were analyzed by linear mixed models depending on soil conditions, field management practices, and climate types. Results Biochar increased the crop yield globally by 14 %, especially in soils with low pH (< 5.5), low nitrogen (N) and phosphorus (P) inputs (≤ 120 kg N ha−1, < 35 kg P ha−1), with high biochar inputs (≥ 20 Mg ha−1), and under crop rotation. Biochar increased crop yields by 12 % in short-term (≤ 5 years) and 21 % in long-term (> 5 years) experiments. Biochar increased yield variability by 42 % in acidic soils (pH < 5.5) and by 24 % with low N inputs (≤ 120 kg N ha−1), mainly because its liming and fertilization effects were short-lasting within the first few years. The yield variability after biochar application decreased with the increase in mean annual temperature, inter-annual variabilities of temperature and precipitation, but with the decrease in mean annual precipitation in the growing season. Yield variability under biochar increased in short-term experiments by 27 %, but there was no change (0 %) in long-term experiments because of the higher yield gains and resistance to fluctuating weather conditions. Therefore, crop yield variability decreased with increasing yield in long-term experiments. Conclusions Biochar application increased short-term variability of crop yields, but its long-term variability remained unaffected. Implications or Significance This study highlights that biochar can support steadily future crop production in the long run.

DOC fluxes were studied within soil profiles on forest toposequences transect of Bukit Dua Belas National Park and Harapan Forest, Jambi, Indonesia. DOC concentration was determined using NPOC (Non Purgeable Organic Carbon) method. Amount and DOC flux from soil horizons on the lower slope was significantly higher than that from the middle and the upper slopes. Amount and DOC flux from AO soil horizon was significantly higher than that from AB and B soil horizons. DOC was maximally accumulated from AO soil horizon of soil profile on lower slope during rainy season.

No abstract is provided for this article.

The Indian mock strawberry [Duchesnea indica (Andrews) Focke] is an invasive plant in several regions of central Europe and Germany. In order to explore it