Synthesis, Conformation Analysis and Biological Evaluation of 2-(2,3-Dideoxy-ß-D-ribofuranosyl)pyridine-4-carboxamide

2-(2,3-Dideoxy-P-~-ribofuranosyl)pyridine-4-carboxamide (2) was synthesized starting from the protected sugar analogue (3).Coupling of this compound with 2-lithio-4-(4,5-dihydro-4,4-dimethyloxazol-2-yl)pyridine (4).subsequent mesylation, reduction, deprotection and ammonolysis gave (2) as an anomeric mixture.&$-Separation was done by semi preparative hplc on a Lichrosorb 10 RP 8 column.In addition 2D-NOESY nmr spectroscopy was used for the assignment of the a/$-anomers of (2).High resolution 'H nmr allowed us to do a conformation analysis of compound (0-2).Finally the p-anomer of (2) was evaluated for its biological activity.