RNAseq libraries preparation, analysis and data processing v1
Preprint 2025 en
Authors
LA
Laura Antonucci
IC
Isidoro Cobo
CN
Chiara Nicoletti
Abstract
1 min read
RNAseq libraries were prepared from mouse pancreata and murine cell lines (CTRL, EZH2KO, EEDKO UN-KC6141) following standard protocols. After RNA extraction and poly-A enrichment, libraries were generated and sequenced on HiSeq 4000 or NextSeq 500 platforms. Reads were aligned to the mm10 genome, and gene expression was quantified and analyzed for differential expression using HOMER, Cufflinks, or DESeq2. Pathway and transcription factor enrichment analyses were performed with GSEA.
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