FAMES analysis v1

Fatty acid methyl ester (FAMES) analysis was used to measure glucose-derived carbon incorporation into fatty acids in KrasG12D/PEC organoids. Organoids were labeled with [U-¹³C₆] glucose, extracted, derivatized, and analyzed by GC-MS. Quantification was done using internal standards and MetaQuant software, allowing assessment of de novo fatty acid synthesis.