Analytical ultracentrifugation (AUC) is the leading technique for determining the molar mass, state of association in solution, and association constants of biological macromolecules. Even though it is little used for supramolecular polymers, this technique has already been shown to represent one of the best options in the characterization of these systems. The use of supramolecular assemblies in the field of nanotechnology requires the arrangement of such systems on surfaces, from solution. Therefore, the control and investigation of solution properties is of major importance. In this contribution we highlight the applicability and advantages of using AUC by presenting a summary of the results of the past few years on the characterization of various types of synthetic polymers and supramolecular polymer systems.
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