Metal-organic framework as a platform of the enzyme to prepare novel environmentally friendly nanobiocatalyst for degrading pollutant in water

Herein, Zeolitic imidazole framework-8 (ZIF-8) as a 3D nanoporous support was synthesized and its surface was functionalized to attain amino-functionalized nanoparticles. Laccase enzyme was covalently immobilized onto ZIF-8 nanoparticles via glutaraldehyde (GA) as the linker to prepare a novel MOF-based nanobiocatalyst (NBC) for degrading organic pollutant (Acid Blue 92: AB92). The FTIR, FESEM, TEM, XRD, BET, and TGA were employed for characterizing of all prepared samples. The effect of catalyst mass, AB92 concentration, temperature, and pH were studied on dye decolorization of. The results showed that the biodegradation amount of AB92 followed by the Michaelis-Menten kinetic model.