<p>Ewing sarcoma is infiltrated by immunosuppressive macrophages and functionally impaired APCs. <b>A,</b> UMAP and dotplot of the myeloid compartment, showing conventional dendritic cells type 1 and 2 (cDC1, cDC2), undifferentiated macrophages (Mo), and differentiated macrophage populations (Mφ) and a selection of their marker genes. <b>B,</b> Macrophage-specific scores for proinflammatory and anti-inflammatory signatures as well as M1 and M2 signatures. <b>C,</b> Antigen presentation and costimulatory capacity score as constructed with genes shown in <a href="#SMF7" target="_blank">Supplementary Fig. S7D</a>. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001 versus Mo. <b>D,</b> Barplot showing percentage of costimulatory gene-expressing Mo, Mφ, and cDCs. Neuroblastoma (NBL; Kildisiute and colleagues) data included as comparative group. Mean percentages and error bars are plotted for individual samples. Samples with <5 cells per group were excluded. <i>*</i>, <i>P <</i> 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001. <b>E,</b> Violin plot depicting DC maturation score for Ewing sarcoma and NBL cDCs. Z-scores were calculated using a curated MSigDB gene set (“LINDSTEDT_DENDRITIC_CELL_MATURATION_B”). This gene set includes upregulated genes both at 8 and 48 hours in response to inflammatory stimuli. ***, <i>P</i> < 0.001. <b>F,</b> Violin plots showing the <i>STAT3</i> and <i>NFKB1</i> gene expression levels of Ewing sarcoma and NBL cDCs. ***, <i>P</i> < 0.001 with Bonferroni correction.</p>
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