FIGURE 1 from Ewing Sarcoma Single-cell Transcriptome Analysis Reveals Functionally Impaired Antigen-presenting Cells

<p>Single-cell architecture of primary Ewing sarcoma. <b>A,</b> Graphical overview of primary patient-derived tissue dissociated and sorted for scRNA-seq. <b>B,</b> Uniform Manifold Approximation and Projection (UMAP) plot colored by patient origin. <b>C,</b> Heat map of top 10 differentially expressed genes per main cell type by average log-normalized expression. <b>D,</b> UMAP with expression of Ewing diagnostic marker (<i>CD99</i>) and a selection of <i>EWS-FLI1</i> candidate target genes (<i>CAV1, CCND1, HES1, KDSR, PAPPA</i>). Dotted line highlights the tumor cell clusters. NA: not expressed. <b>E,</b> Feature plots showing HLA class I module scores (<i>HLA-A, HLA-B, HLA-C, HLA-E, HLA-F, B2M</i>) of main cell types.</p>