Corrigendum to “Daucus carota L. – An old model for cell reprogramming gains new importance through a novel expansion pattern of alternative oxidase (AOX) genes” [Plant Physiol. Biochem. 47 (2009) 753–759] — José Hélio Costa (2014) | RDL Network
Corrigendum to “Daucus carota L. – An old model for cell reprogramming gains new importance through a novel expansion pattern of alternative oxidase (AOX) genes” [Plant Physiol. Biochem. 47 (2009) 753–759]
Corrigendum 2014 English
Authors
JC
José Hélio Costa
HC
Hélia Cardoso
MC
Maria Doroteia Campos
Abstract
1 min read
In the above mentioned paper, the existence of four AOX gene members in Daucus carota was described: two belonging to the AOX1-subfamily (DcAOX1a and DcAOX1b) and two belonging to the AOX2-subfamily (DcAOX2a and DcAOX2b). Given new data, we would like to present here a correction regarding the existence of the DcAOX1b gene.
The authors firstly identified the partial sequences (3′-end) of four AOX genes in D. carota using a PCR approach and degenerated primers as presented in Costa et al. (2009). In subsequent research, the 5′-end of DcAOX1a, DcAOX2a and DcAOX2b were effectively amplified and sequenced, however several attempts failed to detect the 5′-end of AOX1b (cDNA library followed by 5′RACE using different commercial kits and also via chromosome walking). In between, genome analysis was successfully established for carrot through the group of Philipp W. Simon (University of Wisconsin, Madison, USA) and the AOX1b gene was not detected at genome level. Therefore, sequence analysis as well as mapping results confirmed now the existence of only three DcAOX gene members, the previous identified DcAOX1a, DcAOX2a and DcAOX2b (Philipp W. Simon personal communication).
Facing these facts we would like to correct our previous information given on the number of AOX genes in carrot and state that in D. carota only one single AOX1-subfamily member is present, being renamed in NCBI as DcAOX1 (acc. EU286573). Simultaneously the accession number linked to DcAOX1b (acc. EU286574) was removed from NCBI. This knowledge was considered correctly in Costa et al. 2014 (‘A classification scheme for alternative oxidases reveals the taxonomic distribution and evolutionary history of the enzyme in angiosperms’, Mitochondrion, 2014, http://dx.doi.org/10.1016/j.mito.2014.04.007).
The authors apologize for this oversight.
José Hélio Costa, Hélia Cardoso, Maria Doroteia Campos, Maria Amely Zavattieri, António Miguel Frederico, Dirce Fernandes de Melo, Birgit Arnholdt-schmitt
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