Antioxidant and antithrombotic study of novel chitosan-diallyl disulfide inclusion complexes nanoparticles for hemodialysis applications

Chitosan nanoparticles (CNP) is a biopolymer with good antioxidant and blood compatibility. However, its haemostatic nature limits its use for blood-contacting applications. This study aimed to investigate the antioxidant and anti-thrombogenic properties of complexes composed of CNP and diallyl disulfide (DADS). The formation of intramolecular hydrogen bonds proved possible bonding between CNP and DADS to form complexes. XRD spectra showed the complexes were amorphous. The in-vitro study revealed that CNP/DADS exhibits 29.5% NO and 32.8% HP inhibition which proved its antioxidant properties. APTT and PT test results proved that CNP/DADS's antithrombogenic properties (P < 0.001), making it a promising material for hemodialysis application.