The apoptotic effects of natural (n‐) and synthetic (s‐) DADS (diallyl disulfide; 3,3′‐thiobisprop‐1‐ene) on human breast‐cancer MCF‐7 cells were investigated in vitro . 5‐Fu (5‐fluorouracil) and CTX (cyclophosphamide; Cytoxan) were used as comparative control anticancer agents. After MCF‐7 cells had been treated with the drugs, cell viability, morphological change, apoptosis and changes in the cell cycle were measured. The results indicated that s‐ and n‐DADS had similar cytotoxicities towards human breast‐cancer MCF‐7 cells and showed dose‐dependent and time‐dependent inhibitory effects. Morphological observations and flow‐cytometric results showed that the four drugs (s‐DADS, n‐DADS, CTX and 5‐Fu) induced apoptosis in MCF‐7 cells to different extents. The apoptotic effects of s‐ and n‐DADS were superior to those of 5‐Fu and CTX.
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