(A) Lymphocytes of spleen (SP) and inguinal LNs were analyzed for the expression of IgM and IgD by flow cytometry

Numbers indicate the mean percentages and SD of gated populations. SP: follicular (FO) B cells (IgMIgD), marginal zone (MZ), and transitional B cells (IgMIgD); LN: IgMIgD. Values were calculated from seven independent experiments. (B) Flow cytometric analysis of follicular B cells (FO; CD21CD23) and marginal zone B cells (MZ; CD21CD23) in the spleen. Numbers indicate mean percentages and standard deviations of B220 B cells displaying a MZ B or FO B cell phenotype. Values were calculated from seven independent experiments. (C) Absolute numbers of FO and MZ B cells in the spleens of LMP1/CD40 and control mice. (D) Splenocytes were stained with antibodies specific for the indicated activation and adhesion markers. Histograms show surface expression of the indicated markers on gated B220 B cells from LMP1/CD40 (continuous line) and control (dotted line) mice. Lymphocytes isolated from LNs showed a similar extent of activation (not depicted).<b>Copyright information:</b>Taken from "Constitutive CD40 signaling in B cells selectively activates the noncanonical NF-κB pathway and promotes lymphomagenesis"The Journal of Experimental Medicine 2008;205(6):1317-1329.Published online 9 Jun 2008PMCID:PMC2413030.