Publications

<p>Characterisation of Ewing sarcoma tumor cell clusters</p>

(1996). Work and organizational psychology in hospitals. Work & Stress: Vol. 10, No. 3, pp. 193-194.

<p>Supplemental Figure Legends. And Supp Table S1. List of Antibodies used. Table S2. Differential expression of genes in gp130 gastric adenomas compared to wild-type and following deletion of Fzd7.</p>

<p>Figure S5. Frequency of WNT pathway mutations in gastric cancer patients (TCGA).</p>

The objective of this study was to extend the channel model of flow (Csikszentmihalyi, 1975, 1990) by including self-efficacy as predictor of the challenges-skills combination, and of the flow experience itself, based on the predictions of social cognitive theory (Bandura, 1997, 2001). We conducted a two-wave longitudinal study among 258 secondary school teachers. Results, first, showed that the channel model of flow, including self-efficacy as antecedent of flow, fitted better the data. Secondly, it was observed that the more self-efficacy the more flow frequency and higher levels of challenge and skills which, in turn, predicted flow over time. Moreover, the influence of self-efficacy on flow over time was mediated by subjects' perception of the challenges and skills combination. Finally, we discuss the theoretical and practical implications of integrating flow theory into the social cognitive theory.ResumenEl objetivo del presente trabajo es extender el modelo de canal de la experiencia de fluir (flow) (Csikszentmihalyi, 1975, 1990) basándonos en la teoría social cognitiva (Bandura, 1997, 2001) e incluyendo la autoeficacia como predictor de la combinación reto y habilidades, y de la experiencia de fluir (flow). Para ello se llevó a cabo un estudio longitudinal con dos tiempos en una muestra de 258 profesores de educación secundaria. Los resultados mostraron que el modelo que extiende el modelo de canal de la experiencia de fluir (flow), incluyendo la autoeficacia como antecedente del fluir (flow), se ajusta mejor a los datos. Además, se observó que a más autoeficacia más frecuencia de fluir (flow) y mayores niveles de reto y habilidades, que a su vez predecían fluir (flow) en el tiempo. Así, la influencia en el tiempo de las creencias de eficacia en el fluir (flow) estaba mediada por la percepción de la combinación de reto y habilidades. Finalmente se discuten implicaciones teóricas y prácticas de la integración de la teoría de la experiencia de fluir (flow) en la teoría social cognitiva.Keywords: Challengeflow experiencepositive psychologyself-efficacyskillsPalabras clave: Retoexperiencia de flowpsicología positivaautoeficaciahabilidades

The shortage of liver organ donors is increasing and the need for viable alternatives is urgent. Liver cell (hepatocyte) transplantation may be a less invasive treatment compared with liver transplantation. Unfortunately, hepatocytes cannot be expanded in vitro, and allogenic cell transplantation requires long-term immunosuppression. Organoid-derived adult liver stem cells can be cultured indefinitely to create sufficient cell numbers for transplantation, and they are amenable to gene correction. This study provides preclinical proof of concept of the potential of cell transplantation in a large animal model of inherited copper toxicosis, such as Wilson’s disease, a Mendelian disorder that causes toxic copper accumulation in the liver. Hepatic progenitors from five COMMD1-deficient dogs were isolated and cultured using the 3D organoid culture system. After genetic restoration of COMMD1 expression, the organoid-derived hepatocyte-like cells were safely delivered as repeated autologous transplantations via the portal vein. Although engraftment and repopulation percentages were low, the cells survived in the liver for up to two years post-transplantation. The low engraftment was in line with a lack of functional recovery regarding copper excretion. This preclinical study confirms the survival of genetically corrected autologous organoid-derived hepatocyte-like cells in vivo and warrants further optimization of organoid engraftment and functional recovery in a large animal model of human liver disease.

ABSTRACT In mice, intestinal tuft cells have been described as a long-lived, post-mitotic cell type of which two distinct subsets have been identified, named tuft-1 and tuft-2 1 . By combining analysis of primary human intestinal resection material and intestinal organoids, we identify four distinct human tuft cell states, two of which overlap with their murine counterparts. We show that tuft cell development depends on the presence of Wnt ligands, and that tuft cell numbers rapidly increase upon interleukin (IL)-4 and IL-13 exposure, as reported previously in mouse 2–4 . This occurs through proliferation of pre-existing tuft cells, rather than through increased de novo generation from stem cells. Indeed, proliferative tuft cells occur in vivo both in fetal and in adult human intestine. Single mature proliferating tuft cells can form organoids that contain all intestinal epithelial cell types. Unlike stem- and progenitor cells, human tuft cells survive irradiation damage and retain the ability to generate all other epithelial cell types. Accordingly, organoids engineered to lack tuft cells fail to recover from radiation-induced damage. Thus, tuft cells represent a damage-induced reserve intestinal stem cell pool in humans.

Purpose – Work engagement represents an important aspect of employee well-being and performance and has been related to both job and personal resources. The purpose of this paper, based on Social Cognitive Theory, is to emphasize the proactive role of self-efficacy which is hypothesized to predict work engagement, not only directly, but also indirectly through positive changes in employee’s perceptions of social context (PoSC); namely, perceptions of one’s immediate supervisor, colleagues and top management. Design/methodology/approach – A sample of 741 employees of a communication service company completed two questionnaires, with a time interval of three years. Structural equation modeling was performed in order to test the hypothesized model. Findings – Results revealed that, as expected: first, initial self-efficacy predicts work engagement three years later; and second, positive changes in employee’s perceptions of the social work context across the three year period, mediates the relationship between self-efficacy and work engagement. Research limitations/implications – Results relied only upon self-report data. Moreover, each variable was only measured at the time in which it was hypothesized by the conceptual model. Practical implications – The significant role of self-efficacy as a direct and indirect predictor of work engagement suggests the development of training programs centered on the main sources of self-efficacy, specifically focussed on the social work domain. Originality/value – This research provides evidence of the substantial contribution of self-efficacy to work engagement over time. Moreover, the results also support the beneficial effects of self-efficacy through its influence on the improvements in the individuals’ perceptions of their social context.