Publications

The current study extends the Broaden & Build theory to the collective (i.e. groups) level of analysis, focusing on the mediating role of group social resources (i.e. cohesion, coordination, teamwork, supportive team climate) between group positive affect (i.e. enthusiasm, optimism, satisfaction, comfort) and group performance (i.e. in-and extra-role performance, creative performance). To test our hypotheses, we conducted two studies using independent samples. Study 1 is a laboratory study with 449 participants nested in 112 small groups who performed an organizational simulation creative task. Study 2 is a field study that aggregated scores of 2159 employees nested in 417 groups. In both the lab and field studies, structural equation modeling results revealed that group social resources fully mediate the relationship between group positive emotions and performance.

Abstract Atypical teratoid/rhabdoid tumors (ATRTs) represent a rare, but aggressive pediatric brain tumor entity. They are genetically defined by alterations in the SWI/SNF chromatin remodeling complex members SMARCB1 or SMARCA4 . ATRTs can be further classified in different molecular subgroups based on their epigenetic profiles. Although recent studies suggest that the different subgroups have distinct clinical features, subgroup-specific treatment regimens have not been developed thus far. This is hampered by the lack of pre-clinical in vitro models representative of the different molecular subgroups. Here, we describe the establishment of ATRT tumoroid models from the ATRT-MYC and ATRT-SHH subgroups. We demonstrate that ATRT tumoroids retain subgroup-specific epigenetic and gene expression profiles. High throughput drug screens on our ATRT tumoroids revealed distinct drug sensitivities between and within ATRT-MYC and ATRT-SHH subgroups. Whereas ATRT-MYC universally displayed high sensitivity to multi-targeted tyrosine kinase inhibitors, ATRT-SHH showed a more heterogeneous response with a subset showing high sensitivity to NOTCH inhibitors, which corresponded to high expression of NOTCH receptors. Our ATRT tumoroids represent the first pediatric brain tumor organoid model, providing a representative pre-clinical model which enables the development of subgroup-specific therapies.

<p>Interactions literature references</p>

Abstract Breast cancer is characterized by histological and functional heterogeneity, posing a clinical challenge for patient treatment. Emerging evidence suggests that the distinct subtypes reflect the repertoire of genetic alterations and the target cell. However, the precise initiating events that predispose normal epithelium to neoplasia are poorly understood. Here, we demonstrate that breast epithelial organoids can be generated from human reduction mammoplasties (12 out of 12 donors), thus creating a tool to study the clonal evolution of breast cancer. To recapitulate de novo oncogenesis, we exploited clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 for targeted knockout of four breast cancer–associated tumor suppressor genes (P53, PTEN, RB1, NF1) in mammary progenitor cells from six donors. Mutant organoids gained long-term culturing capacity and formed estrogen-receptor positive luminal tumors on transplantation into mice for one out of six P53/PTEN/RB1–mutated and three out of six P53/PTEN/RB1/NF1–mutated lines. These organoids responded to endocrine therapy or chemotherapy, supporting the potential utility of this model to enhance our understanding of the molecular events that culminate in specific subtypes of breast cancer.

In this study we analyze the mediating role of team work engagement between team social resources (i.e., supportive team climate, coordination, teamwork), and team performance (i.e., in-role and extra-role performance) as predicted by the Job Demands-Resources Model. Aggregated data of 533 employees nested within 62 teams and 13 organizations were used, whereas team performance was assessed by supervisor ratings. Structural equation modeling revealed that, as expected, team work engagement plays a mediating role between social resources perceived at the team level and team performance as assessed by the supervisor.

Burnout ist ein stressbedingtes Erschöpfungssyndrom, das im Kontext einer chronischen Stressbelastung am Arbeitsplatz entsteht und einen Risikozustand für psychiatrische und somatische Folgeerkrankungen darstellt.

<p>Table S3</p>

<p>Ewing sarcoma is infiltrated by immunosuppressive macrophages and functionally impaired APCs. <b>A,</b> UMAP and dotplot of the myeloid compartment, showing conventional dendritic cells type 1 and 2 (cDC1, cDC2), undifferentiated macrophages (Mo), and differentiated macrophage populations (Mφ) and a selection of their marker genes. <b>B,</b> Macrophage-specific scores for proinflammatory and anti-inflammatory signatures as well as M1 and M2 signatures. <b>C,</b> Antigen presentation and costimulatory capacity score as constructed with genes shown in <a href="#SMF7" target="_blank">Supplementary Fig. S7D</a>. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001 versus Mo. <b>D,</b> Barplot showing percentage of costimulatory gene-expressing Mo, Mφ, and cDCs. Neuroblastoma (NBL; Kildisiute and colleagues) data included as comparative group. Mean percentages and error bars are plotted for individual samples. Samples with <5 cells per group were excluded. <i>*</i>, <i>P <</i> 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001. <b>E,</b> Violin plot depicting DC maturation score for Ewing sarcoma and NBL cDCs. Z-scores were calculated using a curated MSigDB gene set (“LINDSTEDT_DENDRITIC_CELL_MATURATION_B”). This gene set includes upregulated genes both at 8 and 48 hours in response to inflammatory stimuli. ***, <i>P</i> < 0.001. <b>F,</b> Violin plots showing the <i>STAT3</i> and <i>NFKB1</i> gene expression levels of Ewing sarcoma and NBL cDCs. ***, <i>P</i> < 0.001 with Bonferroni correction.</p>