Publications

To examine the association of maternal physical activity before and during pregnancy with academic performance in youth.This study included 1868 youth (6-18 years) and their mothers. Mothers recalled their physical activity before and during pregnancy. Mothers were categorized into four groups: "remained active", "became inactive", "became active" and "remained inactive". Academic performance was assessed through school records.Boys whose mothers practiced physical activity before or during pregnancy had significantly higher scores in academic performance indicators independently of physical activity, fitness, current body mass index (BMI) and birthweight than those whose mothers did not practice physical activity before or during pregnancy (all p < 0.05). In addition, boys whose mothers remained active had higher scores in all academic indicators (ranging from +0.358 to +0.543) than boys whose mothers remained inactive. Boys whose mothers remained active had higher scores in Language (score +0.546; 95% CI, 0.150-0.940), average of Math and Language (score +0.468; 95% CI, 0.100-0.836) and grade point average (GPA) (score +0.368; 95% CI, 0.092-0.644) than boys whose mothers became active.Maternal physical activity before and during pregnancy may positively influence youth's academic performance. Continuing maternal physical activity practice during pregnancy may have greater benefits for youth's academic performance.

Background: The aim is to study trends in physical activity and TV viewing in seven European countries in the period 1985–2002. Methods: The data are collected through questionnaires in the survey 'Health Behaviour in School-aged Children. A WHO Cross-national study', using nationally representative samples of 11-, 13-, and 15-year-olds. Between 1985/86 and 2001/02, a standard set of items was used to measure vigorous physical activity and TV watching in the study. Austria, Finland, Hungary, Norway, Scotland, Sweden, and Wales used these measures in all surveys. Results: Between 1985/86 and 2001/02, there was a slight increase in Finland in the proportions reporting vigorous physical activity 4 or more times a week, whereas as pattern of stability was observed for the other countries. Across all surveys and countries boys were more likely to report regular vigorous physical activity than girls. No clear pattern emerges when examining trends over time in TV watching. Boys reported spending more time watching TV than girls in all countries. The correlation between the two behaviours at the 1986 and 1998 measurement points was non-significant. Conclusion: The finding that boys were more likely to report regular vigorous physical activity and TV watching confirms results of previous studies. The present study of seven European countries generally indicates stability or a small increase in physical activity of boys and girls aged 11–15 from the mid-1980s to the early 2000s.

No abstract is provided for this article.

In NIH3T3 cells stably transfected with the human c-fos gene, serum, platelet derived growth factor (PDGF), phorbol ester (12-O-tetradecanoyl-phorbol-13-acetate, TPA), ultraviolet irradiation (UV) and 3'-5'-cyclic adenosine monophosphate (cAMP) cause a transient and rapid activation of both the endogenous and the transfected c-fos genes. While serum, TPA, UV and PDGF dependent activation of the gene is severely impaired, when the serum responsive element from position -319 to -300 (SRE, Treisman, 1985) is destroyed, a full response to cAMP is retained. Insertion of a synthetic oligonucleotide corresponding to the SRE element upstream of position -96 restores the responses to TPA and serum, and large parts of the responses to UV and PDGF. The signal transduction chains elicited by UV and TPA are blocked by an inhibitor of protein kinase. Only TPA, however, causes the translocation of protein kinase C to the membrane. UV and TPA treated cells become refractory to a second stimulation by the same agent at 3 or 24 hours after the first treatment. Alternating the agents, however, leads to full responses. In addition, saturating doses of UV and TPA are at least additive. Ca-ionophores severely reduce only UV induced c-fos expression. These data indicate, that different signal transduction pathways elicited by growth promoting agents and by UV induced stress converge onto the same enhancer element.

Microglial cells are activated during excitotoxin-induced neurodegeneration. However, the in vivo role of microglia activation in neurodegeneration has not yet been fully elucidated. To this end, we used Ikkbeta conditional knockout mice (LysM-Cre/Ikkbeta(F/F)) in which the Ikkbeta gene is specifically deleted in cells of myeloid lineage, including microglia, in the CNS. This deletion reduced IkappaB kinase (IKK) activity in cultured primary microglia by up to 40% compared with wild-type (Ikkbeta(F/F)), and lipopolysaccharide-induced proinflammatory gene expression was also compromised. Kainic acid (KA)-induced hippocampal neuronal cell death was reduced by 30% in LysM-Cre/Ikkbeta(F/F) mice compared with wild-type mice. Reduced neuronal cell death was accompanied by decreased KA-induced glial cell activation and subsequent expression of proinflammatory genes such as tumour necrosis factor (TNF)-alpha and interleukin (IL)-1beta. Similarly, neurons in organotypic hippocampal slice cultures (OHSCs) from LysM-Cre/Ikkbeta(F/F) mouse brain were less susceptible to KA-induced excitotoxicity compared with wild-type OHSCs, due in part to decreased TNF-alpha and IL-1beta expression. Based on these data, we concluded that IKK/nuclear factor-kappaB dependent microglia activation contributes to KA-induced hippocampal neuronal cell death in vivo through induction of inflammatory mediators.

RNAseq libraries were prepared from mouse pancreata and murine cell lines (CTRL, EZH2KO, EEDKO UN-KC6141) following standard protocols. After RNA extraction and poly-A enrichment, libraries were generated and sequenced on HiSeq 4000 or NextSeq 500 platforms. Reads were aligned to the mm10 genome, and gene expression was quantified and analyzed for differential expression using HOMER, Cufflinks, or DESeq2. Pathway and transcription factor enrichment analyses were performed with GSEA.

Extracellular stimuli that activate the transcription factor NF-kappaB cause rapid phosphorylation of the IkappaBalpha inhibitor, which retains NF-kappaB in the cytoplasm of nonstimulated cells. Phosphorylation of IkappaBalpha is followed by its rapid degradation, the inhibition of which prevents NF-kappaB activation. To determine the relationship between these events, we mapped the inducible phosphorylation sites of IkappaBalpha. We found that two residues, serines 32 and 36, were phosphorylated in response to either tumor necrosis factor, interleukin-1, or phorbol ester. Substitution of either serine blocks or slows down induction of IkappaBalpha degradation. Substitutions of the homologous sites in IkappaBbeta, serines 19 and 23, also prevent inducible IkappaBbeta degradation. We suggest that activation of a single IkappaB kinas e or closely related IkappaB kinases is the first cr itical step in NF-kappaB activation. Once phosphorylated, IkappaB is ubiquitinated. Unlike wild-type IkappaBalpha, the phosphorylation-defective mutants do not undergo inducible polyubiquitination. As substitution of a conserved lysine residue slows down the ubiquitination and degradation of IkappaBalpha without affecting its phosphorylation, polyubiquitination is required for inducible IkappaB degradation.

This study examined the contributions of home, school, and neighborhood factors related to youth physical activity (PA). Adolescents (ages 12-18; N = 137) and parents of younger children (ages 5-11; N = 104) from three US metropolitan areas completed surveys. Youth PA was estimated from six items assessing overall physical activity. Bivariate analyses between environment factors and PA were significant correlations in each environmental setting for adolescents (r's:0.16-0.28), but for parents of children, only for the home and neighborhood settings (r's: 0.14-0.39). For adolescents, pieces of equipment at home, family recreation membership, equipment at school, and neighborhood aesthetics explained 15.8% of variance in PA. For younger children (based on parent report), pieces of equipment at home, neighborhood traffic safety, walking/cycling facilities, and street connectivity explained 21.4% of the variance in PA. Modifiable factors like increasing access to equipment at home and school, and improving neighborhood aesthetics may impact youth PA. To optimize explanation of youth PA, factors from multiple environments need to be considered.

Background: Interactive health communication technologies have the potential to eliminate or greatly reduce many of the barriers to delivery of preventive services. This paper describes the process of developing and evaluating interactive health communication programs for primary care settings. We present as examples the Patient-centered Assessment and Counseling for Exercise plus Nutrition (PACE+) programs, designed to promote physical activity and healthy nutrition with adolescents and adults. Methods: The PACE+ programs use interactive communication technology to screen multiple behaviors, prioritize areas for intervention, and initiate intervention. Patient information is synthesized for ease of use by clinicians. The patient completes the program on a computer in the clinic waiting room before the provider encounter. Acceptability of the program was evaluated with adolescents (n=252), adults (n=285), and their health care providers. Results: The PACE+ programs were developed, evaluated, modified, and re-evaluated. Feasibility testing indicated that a diverse group of adolescents and adults found the PACE+ computer programs acceptable. Modifications to shorten and refine the programs were identified. Conclusions: Development of interactive health technologies is an iterative process dependent on feedback from intended users and systems of care. Interactive health communication technologies can be incorporated into clinical settings.