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Amid grave concerns over global climate change and with increasingly strained access to fossil fuels, the synthetic biology community has stepped up to the challenge of developing microbial platforms for the production of advanced biofuels. The adoption of gasoline, diesel, and jet fuel alternatives derived from microbial sources has the potential to significantly limit net greenhouse gas emissions. In this effort, great strides have been made in recent years toward the engineering of microorganisms to produce transportation fuels derived from alcohol, fatty acid, and isoprenoid biosynthesis. We provide an overview of the biosynthetic pathways devised in the strain development of biofuel-producing microorganisms. We also highlight many of the commonly used and newly devised engineering strategies that have been employed to identify and overcome pathway bottlenecks and problems of toxicity to maximize production titers.
We have developed a quantitative, empirical method for estimating the age of Type Ia supernovae (SNe Ia) from a single spectral epoch. The technique examines the goodness of fit of spectral features as a function of the temporal evolution of a large database of SNe Ia spectral features. When a SN Ia spectrum with good signal-to-noise ratio over the rest frame range 3800 to 6800 Angstroms \\ is available, the precision of a spectral feature age (SFA) is (1sigma ) ~ 1.4 days. SFA estimates are made for two spectral epochs of SN 1996bj (z=0.574) to measure the rate of aging at high redshift. In the 10.05 days which elapsed between spectral observations, SN 1996bj aged 3.35 +/- 3.2 days, consistent with the 6.38 days of aging expected in an expanding Universe and inconsistent with no time dilation at the 96.4% confidence level. The precision to which individual features constrain the supernova age has implications for the source of inhomogeneities among SNe Ia.
We present a new technique for simulating the time-dependent behavior of an elastic material in general and the nonlinear dynamics of a linearly elastic cable in particular. Results will be presented for both the small displacement of a highly nonlinearly elastic material and the large displacement of a linearly elastic cable. The first simulation, a small displacement of a nonlinearly elastic material, will be used to validate our numerical scheme against a known analytical solution while the second simulation, the large displacement of a linearly elastic cable, will be used to investigate the looping that can occur when wave interactions produce regions of strong local compression.
This chapter outlines the formulation and explicit solution of a number of simple boundary-value problems. Analysis is facilitated by the constraint of incompressibility. Examples include expansionand contraction of cylinders, torsion, azimuthal shear, and cavitation under conditions of spherical symmetry Further examples involving anti-plane shear are discussed in the Problems.
The presence of a comorbid anxiety or mild to moderate depressive disorder did not reduce the efficacy of CBT for insomnia, but it did for its single BT and CT components when used alone. (PsycINFO Database Record
Abstract The Katzman Automatic Imaging Telescope (KAIT) at Lick Observatory is a fully robotic 0.76-m reflector equipped with a CCD imaging camera. Its telescope control system checks the weather, opens the dome, points to the desired objects, finds and acquires guide stars, exposes, stores the data, and manipulates the data without human intervention. There is a 20-slot filter wheel, including UBV RI . Five-minute guided exposures yield detections of stars at R ≈ 20 mag when the seeing is good (≤2″). One of our main goals is to discover nearby supernovae (SNe; redshifts generally less than 5000 km s −1 ), to be used for a variety of studies. Special emphasis is placed on finding them well before maximum brightness. A limit of ~ 19 mag is reached in the 25-sec unfiltered, unguided exposures of our Lick Observatory Supernova Search (LOSS). We can observe over 1200 galaxies in a long night, and we try to cycle back to the same galaxies after 3 to 4 nights. Our software automatically subtracts template images from new observations and identifies supernova candidates that are subsequently examined by student research assistants. LOSS found 20 SNe in 1998, 40 in 1999, and 36 in 2000, making KAIT the world’s most successful search engine for nearby SNe. We also find novae in the Local Group, comets, asteroids, and cataclysmic variables. Multifilter follow-up photometry is conducted of the most important SNe, and all objects are monitored in unfiltered mode. A Web page describing LOSS is at http://astro.berkeley.edu/~bait/kait.html .