Publications

Proteasome activator 28γ (PA28γ) binds to and activates the proteasome in an ATP-independent manner to promote mainly ubiquitin-independent protein degradation in cells. Previously, four transcript variants of PA28γ have been identified, which have been closely correlated with the progression of cancers. In the present study, we predicted the alternative splicing of PA28γ via the bioinformatics tool ASPicDB and 49 splices were predicted. Then, we cloned some new segment according to predication in oral cancer cells using reverse transcription PCR and a novel variant of PA28γ was found. The novel transcript encodes a truncated form compared with other isoforms of PA28γ. However, it contains most of the conserved residues and the 'activation loop' of the PA28γ family. In order to explore its function, we overexpressed the variant in HEK293 cells and demonstrated that this variant is likely to further regulate cell cycle and apoptosis via regulating p53 and the mouse double minute2 homolog (Mdm2).

To investigate the chemical constituents of Clematis aethusifolia,a traditional Mongolian medicine for resolving hard lump.Bioactive guided isolation and purification of Clematis aethusifolia was performed by normal and reverse phase column chromatography, Sephadex LH-20 and preparation HPLC. The structures were identified by 1D,2D-NMR and MS spectral analysis and comparison with literature data. Cytotoxicity of compounds were determined by CCK-8 cell staining method for detecting growth inhibition to five kinds of human solid tumor cell lines.Eight compounds were isolated and identified as vanillicacid( 1),protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4’-O-β-D-glucopyranoside( 4),( +)-syringaresinol-4’-O-β-Dglucopyranoside( 5),7,9,9’-trihydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside( 6),butyl-β-D-fructopyranoside( 7),butyl-β-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity.All the compounds are isolated from this plant for the first time, Results: Eight compounds were isolated and identified as vanillicacid( 1), protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4’-O-β-D-glucopyranoside( 4),( +)-syringaresinol-4’-O-β-Dglucopyranoside( 5),7,9,9’-trihydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside( 6),butyl-β-D-fructopyranoside( 7),butyl-β-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity. compounds 3,6 ~ 8 are isolated from Clematis genus for the first time, compounds 6 and 8 are isolated from Ranunculaceae family for the first time. Compounds 2,8 may be the effective components in Clematis aethusifolia.

In this paper, we present the corrosion behavior of F30S30 concrete used for a bridge cap which was investigated in simulated seawater with 2.0% NaCl, 0.35% MgCl 2 , and 0.25% Na 2 SO 4 under wetdry cycles. This behavior was compared with those in 3.5% NaCl, 5.0% Na 2 SO 4 , and 3.5% NaCl + 5% Na 2 SO 4 solutions. Results show that the change in pore structure obtained through X-ray computed tomography could reflect the amount of corrosion products and the degree of destruction. The variety and quantity of corrosion products varied with the corrosive environment. The damage mechanisms of concrete also changed according to the corrosive environment. The effects of Mg 2+ and SO 4 2- were superimposed on each other for the simulated seawater environment, thereby posing a serious threat to the structure, primarily through damage and coarsening of the pore structure caused by Mg 2+ .

Nitrogen (N) is an essential nutrient for citrus growth and development. Previously, we identified citrus as an ammonium (as NH4 +-N or AN) sensitive plant; however, the mechanism underlying the changes in photosynthetic physiology in response to AN stress remain unclear. ‘Lugan’ (Citrus reticulata Blanco cv. Ponkan) citrus seedlings were sand cultivated in the presence of two forms of N (AN and nitrate as NO3 −-N or NN), and three N levels (1, 4 and 8 mmol L−1, as N1, N4 and N8). Biomass, leaf N concentration per unit leaf area (LNC), gas exchange, relative chlorophyll value (SPAD), photosynthetic N use efficiency (PNUE), chlorophyll a fluorescence, reactive oxygen metabolism, and non-structural carbohydrates (NSC) were determined. Compared with NN, AN significantly inhibited the growth of citrus seedlings, accompanied by a reduced net photosynthetic rate at N4 and N8, as well as LNC at N4, with a concomitant decline in PNUE. Leaf NSC, SPAD, light energy absorption and trapping were remarkably elevated by AN, which damaged the oxygen-evolving complex and increased the heat dissipation, resulting in disruption of the photosynthetic electron transport chain. Moreover, increased superoxide anion, H2O2, and malonaldehyde content, and downregulated superoxide dismutase, peroxidase, and catalase activities were also observed in citrus leaves under AN. However, the severity of these adverse effects of AN in citrus plants increased with N levels. Overall, these results indicate that AN supply reduces PNUE by disrupting the photosynthetic electron transport chain, which inhibits the growth of citrus seedlings.

This study investigates the inhibitory mechanism of anlotinib on human Mantle Cell Lymphoma (MCL) cells through in vitro and in vivo experiments.

This paper presents the feasibility of semi-direct injection on a 50cm 3 , two-stroke motorcycle gasoline engine, which is applied FAI semi-direct injection fuel system. The structure and fuel injection system is improved based on the original carburetor engine and the FAI injector is easily installed. The results of laboratory and drive test show that, compared with the original carburetor fuel system, through optimization calibration of fuel injection timing and injection quantity can improve power performance and fuel economy.

To investigate the protective effect of MZF1/RBM3 on rotenone-induced neuronal injury.Rotenone (1 μM) was used to treat SH-SY5Y cells for 24 hr to simulate the cellular model of Parkinson's disease (PD), followed by detection of SH-SY5Y cell activities using MTT assay. MZF1 expression in rotenone-treated SH-SY5Y cells was detected by qRT-PCR and Western blot. MZF1 overexpression plasmid or MZF1 overexpression plasmid and RBM3 siRNA was transfected into SH-SY5Y cells, and then the expressions of MZF1 and RBM3 were detected. Oxidative stress (OS) in SH-SY5Y cells was detected using CMH2DCF-DA probes. Cell apoptosis rate was detected by flow cytometry. CHIP assay and dual-luciferase reporter assay were used to detect the binding between MZF1 and RBM3 promoter.The expression of MZF1 was significantly lower in the rotenone-induced SH-SY5Y cells. Overexpression of MZF1 significantly reduced OS and apoptosis in rotenone-induced SH-SY5Y cells. MZF1 was a transcription factor of RBM3, which promoted the transcription of RBM3, and knockdown of RBM3 inhibited the protective effect of MZF1 overexpression on SH-SY5Y cells.MZF1 alleviates OS and apoptosis induced by rotenone in SH-SY5Y cells by promoting RBM3 transcription.

The change of external quality,physiological index and environmental gas of sweet cherry at different storage con-ditions was compared.Preservation effect and mechanism of controlled freezing-point and modified atmosphere box preserva-tion on sweet cherry were preliminary studied.Results showed that controlled freezing-point and modified atmosphere box preservation could slow down fruit decay,stalk withering and flesh browning.It also inhibited the increase of MDA content and decrease of soluble solids content,titratable acidity and reducing sugar content.The sweet cherry was preserved better in the modified atmosphere boxes with one old and one new vent opening compared with other treatments.

Boron is an essential microelement for plant growth. Tomato is one of the most cultivated fruits and vegetables in the world, and boron deficiency severely inhibits its yield and quality. However, the mechanism of tomato in response to boron deficiency remains largely unclear. Here, we investigated the physiological and molecular bases of the boron deficiency response in hydroponically grown tomato seedlings. Boron deficiency repressed the expression of genes associated with nitrogen metabolism, while it induced the expression of genes related to the pentose phosphate pathway, thereby altering carbon flow to provide energy for plants to cope with stress. Boron deficiency increased the accumulation of copper, manganese and iron, thereby maintaining chlorophyll content and photosynthetic efficiency at the early stage of stress. In addition, boron deficiency downregulated the expression of genes involved in cell wall organization and reduced the contents of pectin and cellulose in roots, ultimately retarding root growth. Furthermore, boron deficiency markedly altered phytohormone levels and signaling pathways in roots. The contents of jasmonic acid, jasmonoy1-L-isoleucine, trans-zeatin riboside, abscisic acid, salicylic acid, and SA glucoside were decreased; in contrast, the contents of isopentenyladenine riboside and ethylene precursor 1-aminocyclopropane-1-carboxylic acid were increased in the roots of boron-deficient tomato plants. These results collectively indicate that tomato roots reprogram carbon/nitrogen metabolism, alter cell wall components and modulate phytohormone pathways to survive boron deficiency. This study provides a theoretical basis for further elucidating the adaptive mechanism of tomato in response to boron deficiency.