No abstract is provided for this article.
Severe combined immune deficient (SCID) mice inoculated intravenously with vaccinia virus (VV) became sick within 6-8 days and died 10-12 days after infection. Tail lesions developed and the number depended on the virus inoculum. Age-matched immunocompetent NMRI mice similarly infected also developed tail lesions but did not become sick. When the infected SCID mice were treated with the acyclic nucleoside phosphonate HPMPC [(S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine], either for 5 consecutive days starting on the day of infection or for 5 consecutive days starting on day 2, 4, or 6 post infection, or as a single dose at 7 days or 1 day before infection, VV-associated death was significantly delayed. VV-infected SCID mice that received two doses of 20 mg/kg of HPMPC every week survived the infection for about 130 days. The period during which the mice remained disease-free following HPMPC treatment correlated with the absence of detectable virus in their organs. The VV/SCID mouse model employed here may be useful for determining whether (attenuated) recombinant VV (carrying HIV genes) may have detrimental effects in the immunodeficient host. HPMPC may be considered as a drug candidate for the treatment and prophylaxis of such complications.
Le Frittage Selectif par Laser (FSL) est un procede de prototypage rapide prometteur pour la fabrication d'objects 3D fonctionnels par frittage direct de poudres metalliques. Un modele analytique simple a ete developpe pour simuler l'absorption et la penetration de l'energie laser dans le FSL. Ce modele a ete utilise pour des melanges de poudres Fe-Cu et WC-Co, irradiees par lasers Nd-YAG ou CO 2 . Il permet d'evaluer la fraction d'energie totale absorbee et la penetration optique du faisceau laser dans les poudres et de donner une estimation des dimensions de la zone frittee. Les resultats de simulation aideront non seulement a avoir une meilleure connaissance des phenomenes physiques associes mais aussi a optimiser le procede FSL.
SUMMARY The molecular size of poly I.poly C required for lysis of interferon-treated L cells was the same as the size required for induction of interferon in these cells. The size requirement for both interferon induction and toxicity for interferon-treated L cells resided in the poly I strand, while variations of the poly C strand size were without effect on interferon induction or cell lysis. The size of poly I.poly C required to induce interferon in primed L cells or primed primary rabbit kidney cells was the same as the size required to induce interferon in normal L cells or normal rabbit kidney cells. Again, these requirements proved more stringent for the poly I strand than for the poly C strand. These data suggest that both primed and normal cells recognize the same molecular ‘trigger’ for interferon induction.
