Publications

This paper presents a study assessing the effect of development technology choices on the accessibility quality of web pages. We used an off-the-shelf tool to identify technologies over a set of 1669 pages, each from a different domain. We have focused, in particular, in programming languages, Web and JavaScript frameworks, and content management systems categories. Simultaneously, we used an automatic accessibility evaluation tool to assess the accessibility of those pages. The study shows statistical evidence that some technologies lead to more accessible pages, within all categories.

In March 2012 we collected 3-replicated sediment cores (9 cm diameter and 12-15 cm long) per restored site along the planting chronosequence (i.e. years 1994, 1997, 2003, 2004, 2006). Similarly, we collected 3 sediment cores in two bare sites, previously colonized by seagrasses, and in a large seagrass patch that survived the disturbances in the second half of the 20th Century that we considered a mature and thus reference meadow. We measured sediment bulk density and organic matter content and carbon stocks along all sediment cores sliced at 1 cm interval. Organic carbon content (% Corg) was estimated from loss of ignition (% LOI) at 550oC for 5 h using the empirically fitted equation for Oyster Harbour sediments,Log % Corg = -0.62 + (1.33 * Log % LOI) SEintercept = 0.01, SEslope =0.10, N = 55, R2 = 0.77, P<0.0001 We analyzed the ƌ3C of the organic carbon in the top 3 cm sediment layer (ƌ13Csediment) along the chronosequence to estimate the fraction of seagrass (X) and sestonic (1-X) deposition as ƌ13Csediment = [X . Log % Corg = -0.62 + (1.33 * Log % LOI) SEintercept = 0.01, SEslope =0.10, N = 55, R2 = 0.77, P<0.0001 We analyzed the ƌ3C of the organic carbon in the top 3 cm sediment layer (ƌ13Csediment) along the chronosequence to estimate the fraction of seagrass (X) and sestonic (1-X) deposition as ƌ13Csediment = [X . ƌ13Cseagrass] + [(1-X) . ƌ13Cseston] being ƌ13Cseagrass -9.65 ‰ and ƌ13Cseston -22 ‰ (Dauby 1989).

Abstract Anthropogenic sea level rise (SLR) presents one of the greatest risks to human lives and infrastructures. Coastal vegetated ecosystems, that is, tidal marshes, seagrass meadows, and mangrove forests, elevate the seabed through soil accretion, providing a natural coastline protection against SLR. The soil accretion of these ecosystems has never been assessed in hot desert climate regions, where water runoff is negligible. However, tropical marine ecosystems are areas of intense calcification that may constitute an important source of sediment supporting seabed elevation, compensating for the lack of terrestrial inputs. We estimated the long‐term ( 14 C‐centennial) and short‐term ( 210 Pb‐20th century) soil accretion rates (SARs) and inorganic carbon (C inorg ) burial in coastal vegetated ecosystems of the Saudi coasts of the central Red Sea and the Arabian Gulf. Short‐term SARs (±SE) in mangroves of the Red Sea (0.27 ± 0.22 cm/year) were twofold the SLR for that region since 1925 (0.13 cm/year). In the Arabian Gulf, only mangrove forest SAR is equivalent to local SLR estimates for the period 1979–2007 (0.21 ± 0.09 compared to 0.22 ± 0.05 cm/year, respectively). Long‐term SARs are comparable or higher than the global estimates of SLR for the late Holocene (0.01 cm/year). In all habitats of the Red Sea and Arabian Gulf, SARs are supported by high carbonate accretion rates, comprising 40% to 60% of the soil volume. Further studies on the role of carbonates in coastal vegetated ecosystems are required to understand their role in adaptation to SLR.

Abstract Giant clams (Tridacninae) are important members of Indo‐Pacific coral reefs and among the few bivalve groups that live in symbiosis with unicellular algae (Symbiodiniaceae). Despite the importance of these endosymbiotic dinoflagellates for clam ecology, the diversity and specificity of these associations remain relatively poorly studied, especially in the Red Sea. Here, we used the internal transcribed spacer 2 (ITS2) rDNA gene region to investigate Symbiodiniaceae communities associated with Red Sea Tridacna maxima clams. We sampled five sites spanning 1,300 km (10° of latitude, from the Gulf of Aqaba, 29°N, to the Farasan Banks, 18°N) along the Red Sea's North‐South environmental gradient. We detected a diverse and structured assembly of host‐associated algae with communities demonstrating region and site‐specificity. Specimens from the Gulf of Aqaba harbored three genera of Symbiodiniaceae, Cladocopium, Durusdinium , and Symbiodinium , while at all other sites clams associated exclusively with algae from the Symbiodinium genus. Of these exclusively Symbiodinium ‐associating sites, the more northern (27° and 22°) and more southern sites (20° and 18°) formed two separate groupings despite site‐specific algal genotypes being resolved at each site. These groupings were congruent with the genetic break seen across multiple marine taxa in the Red Sea at approximately 19°, and along with our documented site‐specificity of algal communities, contrasted the panmictic distribution of the T. maxima host. As such, our findings indicate flexibility in T. maxima ‐Symbiodiniaceae associations that may explain its relatively high environmental plasticity and offers a mechanism for environmental niche adaptation.

Abstract Estimates of marine plastic stocks, a major threat to marine life (1), are far lower than expected from exponentially-increasing litter inputs, suggesting important loss factors (2, 3). These may involve microbial degradation, as the plastic-degrading polyethylene terephthalate enzyme (PETase) has been reported in marine microbial communities (4). An assessment of 416 metagenomes of planktonic communities across the global ocean identifies 68 oceanic PETase variants (oPETase) that evolved from ancestral enzymes degrading polycyclic aromatic hydrocarbons. Twenty oPETases show predicted efficiencies comparable to those of laboratory-optimized PETases, suggesting strong selective pressures directing the evolution of these enzymes. We found oPETases in 90.1% of samples across all oceans and depths, particularly abundant at 1,000 m depth, with a strong dominance of Pseudomonadales containing putative highly-efficient oPETase variants in the dark ocean. Enzymatic degradation may be removing plastic from the marine environment while providing a carbon source for bathypelagic microbial communities.

Environmental variables (salinity, depth, and temperature) in control and fertilized plots during the experiment.

Recent research has highlighted the valuable role that coastal and marine ecosystems play in sequestering carbon dioxide (CO2). The carbon (C) sequestered in vegetated coastal ecosystems, specifically mangrove forests, seagrass beds, and salt marshes, has been termed "blue carbon". Although their global area is one to two orders of magnitude smaller than that of terrestrial forests, the contribution of vegetated coastal habitats per unit area to long-term C sequestration is much greater, in part because of their efficiency in trapping suspended matter and associated organic C during tidal inundation. Despite the value of mangrove forests, seagrass beds, and salt marshes in sequestering C, and the other goods and services they provide, these systems are being lost at critical rates and action is urgently needed to prevent further degradation and loss. Recognition of the C sequestration value of vegetated coastal ecosystems provides a strong argument for their protection and restoration; however, it is necessary to improve scientific understanding of the underlying mechanisms that control C sequestration in these ecosystems. Here, we identify key areas of uncertainty and specific actions needed to address them.

Abstract The hadopelagic environment remains highly understudied due to the inherent difficulties in sampling at these depths. The use of sediment environmental DNA (eDNA) can overcome some of these restrictions as settled and preserved DNA represent an archive of the biological communities. We use sediment eDNA to assess changes in the community within one of the world's most productive open‐ocean ecosystems: the Atacama Trench. The ecosystems around the Atacama Trench have been intensively fished and are affected by climate oscillations, but the understanding of potential impacts on the marine community is limited. We sampled five sites using sediment cores at water depths from 2400 to ~8000 m. The chronologies of the sedimentary record were determined using 210 Pb ex . Environmental DNA was extracted from core slices and metabarcoding was used to identify the eukaryote community using two separate primer pairs for different sections of the 18S rRNA gene (V9 and V7) effectively targeting pelagic taxa. The reconstructed communities were similar among markers and mainly composed of chordates and members of the Chromista kingdom. Alpha diversity was estimated for all sites in intervals of 15 years (from 1842 to 2018), showing a severe drop in biodiversity from 1970 to 1985 that aligns with one of the strongest known El Niño events and extensive fishing efforts during the time. We find a direct impact of sea surface temperature on the community composition over time. Fish and cnidarian read abundance was examined separately to determine whether fishing had a direct impact, but no direct relation was found. These results demonstrate that sediment eDNA can be a valuable emerging tool providing insight in historical perspectives on ecosystem developments. This study constitutes an important step toward an improved understanding of the importance of environmental and anthropogenic drivers in affecting open and deep ocean communities.