Publications

A novel strategy was developed for microRNA detection based on the fluorescence quenching of polydopamine (PDA)-coated reduced graphene oxide (RGO) nanosheets (RGO@PDA). Compared with graphene oxide (GO), the reduction of GO and modification of the surface of RGO by PDA not only improve the stability, dispersity, biocompatibility, and cellular uptake without degeneration of the unique electronic properties of graphene, but also add an electron gate for harvesting electrons, as well as enabling efficient and forward electron transfer to avoid unwanted electron transfer and realize highly sensitive miRNA detection; thus a lower detection limit can be achieved in this sensing system. Remarkably, nanoprobes consisting of RGO@PDA and fluorescein-labeled single-stranded DNA can naturally enter cancer cells without the aid of transfection agents, as well as resisting enzymatic lysis and showing almost no effect on the cell viability. More importantly, intense and time-dependent fluorescence responses were observed from the important tumor marker microRNA-21 (miR-21) in living cells; thus suggesting that the proposed sensing platform shows great promise for applications in disease diagnosis and fundamental research into biochemistry.

Fullerene-based organic compounds have been reported as useful materials with some limitations; nonetheless, fullerene-free compounds are primarily considered to be the most substantial materials for the development of modern technology. Therefore, in this study, a series of compounds (<b>NFBC2</b>-<b>NFBC7</b>) having an A<i>-</i>π-D architecture were designed for the first time from a synthesized nonfullerene (<b>O-IDTBR</b>) compound by changing different acceptor groups. The synthesized nonfullerene (<b>O-IDTBR1</b>) compound and its designed derivatives were optimized with frequency analyses at the M06/6-311G(d,p) level. These optimized structures were further characterized by different quantum chemical approaches. The study required that the designed compounds possess a low energy gap in comparison to that of <b>O-IDTBR1</b> (2.385 eV). Moreover, density of state (DOS) calculations supported the FMO analysis and displayed charge transfers from the HOMO to the LUMO in an effective manner. The λ_max values of the investigated chromophores were observed to be greater than that of the reference compound. Amazingly, the highest amplitude of linear polarizability ⟨α⟩ and first (β_tot) and second hyperpolarizability values were achieved by <b>NFBC6</b> at 1956.433, 2155888.013, and 7.868 × 10⁸ au, respectively, among all other derivatives. Effective NLO findings revealed that nonfullerene-based derivatives may contribute significantly to NLO technology.

Quantum dots (QDs) are semiconducting inorganic nanoparticles, tiny molecules of 2-10 nm sizes to strength the quantum confinements of electrons. The QDs are good enough to emit light onto electrons for exciting and returning to the ground state. Here, CdSe/ZnS core/shell QDs have been prepared and applied for electrochemical sensor development in this approach. Flat glassy carbon electrode (GCE) was coated with CdSe/ZnS QDs as very thin uniform layer to result of the selective and efficient sensor of 3-CP (3-chlorophenol). The significant analytical parameters were calculated from the calibration plot such as sensitivity (3.6392 µA µM^-1 cm^-2) and detection limit (26.09 ± 1.30 pM) with CdSe/ZnS/GCE sensor probe by electrochemical approach. The calibration curve was fitted with the regression co-efficient r² = 0.9906 in the range of 0.1 nM ∼ 0.1 mM concentration, which denoted as linear dynamic range (LDR). Besides these, it was performed the reproducibility in short response time and successfully validated the fabricated sensor for 3-CP in the real environmental and extracted samples. It is introduced as a noble route to detect the environmental phenolic contaminants using CdSe/ZnS QDs modified sensor by electrochemical method for the safety of healthcare and environmental fields at broad scales.

The human nasopharynx-associated lymphoid tissue (NALT) and bronchus-associated lymphoid tissue (BALT) could successfully limit the spread of several respiratory pathogens. However, in the case of MERS-CoV infection, limited research has been conducted in humans with regard to immunopathogenesis and mucosal immune responses due to the lack of relevant tissues. A better understanding of the immune mechanisms of the URT and LRT is vital for the design and development of effective MERS-CoV vaccines.

Three novel derivatives of (<i>E</i>)-<i>N</i>'-nitrobenzylidene-benzenesulfonohydrazide (NBBSH) were synthesized by a condensation method from nitrobenzaldehyde and benzenesulfonylhydrazine reactants in low to moderate yields, which crystallized in methanol, acetone, ethyl acetate, and ethanol. NBBSH derivatives were totally characterized using various spectroscopic techniques, such as Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, proton nuclear magnetic resonance spectroscopy (¹H NMR), and carbon-13 nuclear magnetic resonance (¹³C NMR) spectroscopy. The molecular structure of the NBBSH derivates was confirmed by the single crystal X-ray diffraction method and used for potential detection of a selective heavy metal ion, mercury (Hg²⁺), by a reliable <i>I</i>-<i>V</i> method. A thin coating of NBBSH derivatives was deposited on a glassy carbon electrode (surface area = 0.0316 cm²) with a binder (nafion) coating to modify a sensitive and selective Hg²⁺ sensor with a short response time in phosphate buffer. The modified cationic sensor exhibited enhanced chemical performances, such as higher sensitivity, linear dynamic range, limit of detection (LOD), reproducibility, and long-term stability toward Hg²⁺. The calibration curve was found to be linear over a wide range of Hg²⁺ concentrations (100.0 pM-100.0 mM). The sensitivity and LOD were considered to be ∼949.0 pA μM^-1cm^-2 and 10.0 ± 1.0 pM (S/N = 3), respectively. The sensor was applied to the selective measurement of Hg²⁺ in spiked water samples to give acceptable and satisfactory results.