1,210 publications from this institution
The aim of the study was to investigate the ability of Zea mays L. roots to regulate the amount of free amino acids present in the rhizosphere. The active uptake of amino acids was shown to conform to Michaelis-Menten kinetics. Comparison of amino acid-N and NO3-N...
A sample of 38 southern peaked-spectrum radio sources from the Parkes Catalogue have been observed using single-baseline VLBI. Thirty three objects were successfully detected on baselines of > 30 M λ at 2.3 GHz. For 21 of these sources, the flux density in the compact components contributes more than half the total flux density of the radio emission. Twenty sources showed structure more complex than a point-source.
Bacterial vaginosis (BV) is a prevalent condition associated with an imbalance in the vaginal microbiota, often involving species of Gardnerella . The taxonomic complexity and inconsistent nomenclature of Gardnerella have impeded progress in understanding the role of specific species in health and disease. In this study, we conducted a comprehensive genomic and pangenomic analysis to resolve taxonomic ambiguities and elucidate metabolic and virulence potential across Gardnerella species. We obtained complete, closed genomes for 42 Gardnerella isolates from women with BV and curated publicly available genome sequences (n = 291). Average nucleotide identity (ANI) analysis, digital DNA-DNA hybridization (dDDH), and the cpn60 gene sequences identified nine species and eleven subspecies within Gardnerella , for which we refined species and subspecies boundaries and proposed updated nomenclature. Pangenome analysis revealed species-specific gene clusters linked to metabolic pathways, virulence factors, and niche adaptations, distinguishing species specialized for mucin degradation in the vaginal environment from those potentially adapted to urinary tract colonization. Notably, we identified lineage-specific evolutionary divergence in gene clusters associated with biofilm formation, carbohydrate metabolism, and antimicrobial resistance. We further discovered the first cryptic plasmids naturally present within the Gardnerella genus. Our findings provide a unified framework for Gardnerella taxonomy and nomenclature, and enhance our understanding of species-specific functional capabilities, with implications for Gardnerella research, diagnostics, and targeted therapeutics in BV.
The direct uptake of dissolved organic nitrogen (DON) by plants has the potential to be a primary Factor in ecosystem functioning and vegetation succession particularly in N-limiting environments. Clear experimental evidence to support this view, however, is still lacking. Further, many of the experimental approaches used to assess whether DON is important may be compromised due to the use of inappropriate methods for comparing and quantifying plant available inorganic and organic soil N pools. In addition, experiments aimed at quantifying plant DON capture using dual-labelled (15N, 13C) organic N tracers often do not consider important aspects such as isotope pool dilution, differences in organic and inorganic N pool turnover times, bi-directional DON flows at the soil–root interface, and the differential fate of the 15N and 13C in the tracer compounds. Based upon experimental evidence, we hypothesize that DON uptake from the soil may not contribute largely to N acquisition by plants but may instead be primarily involved in the recapture of DON previously lost during root exudation. We conclude that while root uptake of amino acids in intact form has been shown, evidence demonstrating this as a major plant N acquisition pathway is still lacking.
The release of organic acid anions from plant roots into soil has been hypothesized to be a mechanism for enhancing phosphorus availability in the rhizosphere. Although these compounds are excreted from the cytoplasm as organic acid anions (e.g. citrate, malate), when the H+-ATPase is also upregulated there is evidence to suggest that they enter the soil as organic acids (e.g. citric acid, malic acid). The aim of this study was to evaluate the role of citric acid (H-citrate) and potassium citrate (K-citrate) in the mobilization and plant uptake of P from two acid soils contrasting in their P availability. Our results indicated that the mobilization of P from a KH 2 33 PO 4 labelled patch of soil was soil type dependent, was controlled by its intrinsic P status, and that more P was made available by K-citrate than H-citrate. Similarly, the uptake of 33P from the rhizosphere by Zea mays L. was greatest in the presence of K-citrate in comparison to H-citrate. However, a significant increase in shoot 33P content was only observed in the more acidic soil with high P sorption potential (Haplic podzol) while no significant increase was observed in the less acidic soil with low P sorption potential (Eutric cambisol). We conclude that the chemical form of organic acid anion excretion may have a significant impact on its P mobilization capability. The contrasting results with the two acid soils indicate that organic acids may not provide a universal mechanism for enhancing P uptake from soil.
Birefringent clusters of crystals have been found in the cortex of root nodules in Alnus glutinosa during a scanning electron microscopy study of cryofixed material. Such nodules are formed by nitrogen-fixing actinomycetes of the genus Frankia. The crystals were soluble in hydrochloric acid, insoluble in acetic acid and gave calcium only on energy dispersive X-ray micro-analysis. The evidence suggested that the crystals were composed of calcium oxalate. This appears to be the first report of oxalate in nitrogen-fixing nodules of non-leguminous species.
Salt accumulation and salinisation of coastal soils is a global issue. Further, climate change is likely to increase the amount of land affected by salinity due to the increasing frequency and severity of coastal flooding and brackish water ingress. The impact of this on the ability of soils to deliver ecosystem services, particularly carbon (C) storage, however, remains unclear. We hypothesized that coastal inundation would negatively affect C storage by lowering plant C inputs and by placing greater osmotic stress on the microbial community leading to a reduced C use efficiency (CUE). Here, we use a coastal grassland ecosystem, which is becoming increasingly subjected to sea and brackish water flooding, to explore the relationship between plant/microbial growth and CUE along a natural salinity gradient. To reflect steady state conditions, we traced the turnover and partitioning of a low (ambient) dose and high (growth stimulation) dose of 14C-labelled glucose into microbial anabolic and catabolic pools, from which microbial CUE was calculated. This was supported by measurements of the diversity of the bacterial community across the salinity gradient using 16S metabarcoding. Our results showed that coastal flooding significantly reduced plant growth (p < 0.005), increased soil C content (p < 0.05) and induced an increase in microbial CUE under low glucose-C conditions (p < 0.05). Conversely, no significant difference in CUE or microbial growth was apparent when a high glucose-C dose was used. Soil bacterial community alpha (α) diversity increased with soil salinity while beta (β ) diversity also shifted in response to the higher saline conditions. Our analysis suggests that the largest impact of coastal flooding on soil C cycling was the inability of the plant community to adapt, leading to higher plant residue inputs as well as the decline in soil structure. Conversely, the microbial community had adapted to the increased salinity, resulting in only small changes in the uptake and metabolic partitioning of C.
