Abstract
2 min readBotrytis grey mould (BGM) is a potential threat to chickpea production in Australia owing to favourable environmental conditions and its wide host range. Identifying durable and stable resistant sources against the prevailing virulences of the pathogen is a pre-requisite in the development of disease resistant cultivars. The present study investigated morpho-cultural and pathogenic variation in 22 isolates of B. cinerea isolated from six different hosts at various locations in Western Australia. The isolates varied in colony colour and size; colour, number and size of sclerotia; and sporulation. Based on the differential disease reaction in a set of arbitrarily selected 34 differential lines, the isolates were categorised into distinct pathotypes. Isolate 210.99 from chickpea, with virulence on 33 genotypes, was the most virulent. It was followed by isolates 209.99 & 649.4; 678.2 & 648.13; 214.99, 673 & 619; and 617.99 with virulence on 24, 23, 22, and 21 genotypes, respectively. Lathyrus isolate 216.99, with susceptible reaction on one genotype only, was the least virulent, followed by isolates 97-95, 671, 97-95-1, 651.4 & 651.4, 616.1, and 615.5 with virulence on 3, 5, 6, 8 and 9 genotypes, respectively. In general, isolates from lathyrus and vetch were the least virulent. None of the genotypes showed resistance to all the 22 isolates. Genotypes FLIP 97-548, ICC 14912, ICC 6098 and ICC 4983 showed resistance to 20, 18, 17 and 16 isolates, respectively. Genotypes Heera, ICC 17382, 97-132B189-99V4002 and FLIP 97-539 showed resistance to 15 isolates each. The study revealed that B. cinerea populations from Western Australia were highly variable. Isolates from lentil, lathyrus and weeds such as double gee cross-infected chickpea genotypes may serve as an important alternative host for a source of inoculum for chickpea crop. Further, Some chickpea genotypes FLIP 97- 539, FLIP 97-548, ICC 14912 and ICC 4093 showed multi-pathotype and multiple resistance to B. cinerea and Ascochyta rabiei, which may serve as useful donors in breeding programs. . KEYWORDS :Botrytis cinerea, chickpea, morpho-cultural variation, pathogenic variation, resistance, Virulence
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