Uniform Amplification of Phage with Different Growth Characteristics in Individual Compartments Consisting of Monodisperse Droplets

Save every clone! In phage display, clones displaying ligands that hinder growth of phage are lost in amplification. Competition of slowly (S) and rapidly (R) growing phage is mitigated in monodisperse emulsions generated by a simple microfluidic device. Separating R and S in ca. 107 droplets maintains R/S ratio throughout amplification. Competition-free amplification of phage preserves ligands that are usually lost in phage display screen. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.