Two-photon-induced fluorescence is observed from the photodynamic phycobiliprotein phycoerythrin. Temporal, spectral, and intensity-dependent properties of the two-photon-induced fluorescence emission from phycoerythrin excited by a 1.06-mum laser beam are reported. The measured two-photon absorption cross section of phycoerythrin is an order of magnitude larger than that of Rhodamine 6G. The potential applications of phycobiliproteins for two-photon-induced fluorescence for microscopy of three-dimensional biological samples and three-dimensional optical memory are discussed.
Zujin Zhao, Bin Chen, Junlong Geng, Zhengfeng Chang, Laura Aparicio‐Ixta, Han Nie, Chi Ching Goh, Lai Guan Ng, Anjun Qin, Gabriel Ramos‐Ortíz, Bin Liu, Ben Zhong Tang
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