Two-photon Imaging of Intracellular Hydrogen Peroxide with a Chemoselective Fluorescence Probe

Hengchang Guo; Hossein Aleyasin; Scott S. Howard; Bryan C. Dickinson; Renee Haskew-Layton; Demirhan Kobat; Vivian Lin; David R. Rivera; Christopher J Chang; Rajiv R. Ratan; Chris Xu

doi:10.1364/omp.2011.otud3

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Two-photon Imaging of Intracellular Hydrogen Peroxide with a Chemoselective Fluorescence Probe

Article 2011 en

Abstract

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We present two-photon imaging of hydrogen peroxide (H2O2) production in mouse hippocampal neuronal cell line (HT22) using Peroxyfluor-6 acetoxymethyl ester (PF6-AM), a highly sensitive, small-molecule probe for selective imaging of H2O2 within the living cells.

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