Two Methods Detect the Tyrosine Phosphorylation of STAT3

Objective FCM(flow cytometry) is compared with Western blot in investigating the phosphorylated of STAT3.Methods Promyelocytic leukemia cell line HL-60 are cultured and stimulate with human IL(hIL)-5 in different concentration and then are detected with Western Blot and FCM simultaneously.Results The results of Western blot and FCM are identical.Conclusion The two methods are all have their own advantages in investigating phosphorylated STAT3 and they are mutually complementary.