Thymidylate synthetase‐positive and ‐negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors

Jan Balzarini; De Clercq Erik; Dai Ayusawa; Tetsuzo Seno

doi:10.1016/0014-5793(84)81052-2

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De Clercq Erik

KU Leuven

Thymidylate synthetase‐positive and ‐negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors

Article 1984 en

Authors

JB
Jan Balzarini
De Clercq Erik
KU Leuven
DA
Dai Ayusawa

Abstract

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The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/TS- cells, and their inhibitory effects on FM3A/O cell growth should be readily reversed by exogenous dThd within the concentration range of 5-20 microM.

Thymidylate synthetase‐positive and ‐negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors

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