Thymidylate synthetase‐positive and ‐negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors

Jan Balzarini; De Clercq Erik; Dai Ayusawa; Tetsuzo Seno

doi:10.1016/0014-5793(84)81052-2

Back

Thymidylate synthetase‐positive and ‐negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors

Article 1984 en

Abstract

1 min read

The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/TS- cells, and their inhibitory effects on FM3A/O cell growth should be readily reversed by exogenous dThd within the concentration range of 5-20 microM.

Discussion(0)

No comments yet. Be the first to comment.

Related publications

Article1984

Thymidylate synthetase-deficient mouse FM3A mammary carcinoma cell line as a tool for studying the thymidine salvage pathway and the incorporation of thymidine analogues into host cell DNA

Jan Balzarini, De Clercq Erik, Dai Ayusawa, Tetsuzo Seno

Article1985

Incorporation of 5-substituted pyrimidine nucleoside analogues into DNA of a thymidylate synthetase-deficient murine FM3A carcinoma cell line.

Jan Balzarini, De Clercq Erik, Dai Ayusawa, Tetsuzo Seno

Article1985

Establishment of mutant FM3A murine mammary carcinoma cell strains transformed with the herpes simplex virus type 1 thymidine kinase gene.

Dai Ayusawa, Kimiko Shimizu, Takeshi Seno, Jan Balzarini, De Clercq Erik

Article1986

Establishment of mutant murine mammary carcinoma FM3A cell strains transformed with the herpes simplex virus type 2 thymidine kinase gene.

Kimiko Shimizu, Lisa Ren, Dai Ayusawa, Takeshi Seno, Jan Balzarini, De Clercq Erik

Article1987

Increased sensitivity of thymidine kinase-deficient (TK-) tumor cell lines to the cell growth inhibitory effects of (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) and related compounds.

Jan Balzarini, De Clercq Erik, A. Verbruggen, Clyde S. Crumpacker, Dai Ayusawa, Tetsuzo Seno