The Studies of Allicin on Induction of the Apoptosis in Human Laryngeal Squamous Cell Carcinoma Strain Hep-2

Objective To study apoptosis-inducing activity of allicin in human laryngeal squamous cell carcinoma strain Hep-2.Methods Using techniques of tumor cells culture in vitro Hep-2 cells were exposed to different concentration allicin as 12.5,25.0,50.0,100.0μg /ml,growth characteristics of Hep-2 cells were studied by light microscope,MTT assay and flow cytometry.Results In normal contrast group.Hep-2 cells grew intensively and contacted with each other.However,cells which were treated with lower dose allicin were declined greatly.In higher dose allicin group,necrosis could be found.MTT assay showed that allicin inhibited growth of laryngeal cell with dose and time dependence manner.Flow cytometry showed that allicin could block cell growth at G0/G1 phase,inducing apoptosis of laryngeal cell.Conclusions Human laryngeal squamous cell carcinoma strain Hep-2 can be inhibited and induced into apoptosis by treated with allicin.