Supplementary Figs. S1-S8 and Table S1 from RIP1 Kinase Is an Oncogenic Driver in Melanoma

<p>Supplementary Figs. S1-S8 and Table S1 Supplementary Materials and Methods - The primers used for qPCR. Supplementary Fig. S1. Quantitation of RIP1 expression in melanocytic tumors. Supplementary Fig. S2. Melanocytes of different origins express lower levels of RIP1 than melanoma cells. Supplementary Fig. S3. Overexpression of RIP1 triggers cell death independently of its kinase domain. Supplementary Fig. S4. NF-kB is constitutively activated in melanoma cells. Supplementary Fig. S5. RIP1 overexpression triggers moderate reduction in melanocyte viability independently of caspases. Supplementary Fig. S6. RIP1 mRNA expression and its gene copy number variations in fresh melanoma isolates. Supplementary Fig. S7. The turnover rates of RIP1 mRNA are comparable between melanoma cells and melanocytes. Supplementary Fig. S8. Overexpression of RIP1 reverses reduction in NF-kB activation and melanoma cell proliferation caused by knockdown of TNFR1. Supplementary Table S1. Summary of melanocytic tumors and their expression levels of RIP1.</p>