Stop‐transfer function of pseudo‐random amino acid segments during translocation across prokaryotic and eukaryotic membranes

We have measured the efficiency of stop‐transfer function for a set of pseudo‐random, 18‐residue amino acid segments, both in Escherichia coli and in mammalian microsomes. In general, stop‐transfer function correlates well with the mean hydrophobicity of the segment, though exceptions exist. Kinetic studies suggest that polar segments are rapidly translocated through the E. coli inner membrane and that strongly hydrophobic segments become permanently anchored, while sequences with an intermediate mean hydrophobicity become partly trapped in a transmembrane disposition for a considerable time before being released to the periplasm or degraded.