Spurious Amplification of a <i>Plasmodium vivax</i> Small-Subunit RNA Gene by Use of Primers Currently Used To Detect <i>P. knowlesi</i> — Mallika Imwong (2009) | RDL Network
Spurious Amplification of a <i>Plasmodium vivax</i> Small-Subunit RNA Gene by Use of Primers Currently Used To Detect <i>P. knowlesi</i>
Journal of Clinical Microbiology 47(12): 4173-4175
Article 2009 English
Authors
MI
Mallika Imwong
NT
Naowarat Tanomsing
SP
Sasithon Pukrittayakamee
Abstract
1 min read
The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.
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Koukeo Phommasone, Frank van Leth, Mallika Imwong, Gisela Henriques, Tiengkham Pongvongsa, Bipin Adhikari, Thomas J. Peto, Cholrawee Promnarate, Mehul Dhorda, Pasathorn Sirithiranont, Mavuto Mukaka, Pimnara Peerawaranun, Nicholas Day, Frank Cobelens, Arjen M. Dondorp, Paul N. Newton, Sir Nicholas White, Lorenz von Seidlein, Mayfong Mayxay
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