Abstract The IO− 3 anion gives a redox reaction, in hydrochloric acid medium, with 1,2,4-trihydroxyanthraquinone-3-carboxylic acid (Pseudopurpurin), which shows adequate analytical fluorescent characteristics. The oxidation product produces a fluorescent emission at 625 nm (wavelength of excitation =515 nm). After studying many variables, as a conclusion, we propose a new spectrofluorimetric method for the determination of IO− 3 between 0.2 and 2.0 ppm with a 0.90 % relative error. The interference levels, stoichiometry and nature of reaction are studies.
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