In the present study chromosome numbers of 13 Trifolium species were determined, making a total of 155 species for which chromosome numbers have been reported. Four species which have been reported only once were confirmed. Eleven of the 17 species observed have 16 chromosomes (n = 8). T. bocconei Savi was found to have 12 chromosomes (n = 6) and T. desvauxii Boess. and Bl. was found to have 10 chromosomes (n = 5). Karyotype analyses of T. bocconei and T. desvauxii revealed that the chromosomes of each species could be differentiated on the basis of arm ratios and chromosome lengths.
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