Separation of Hydrophilic Oligomers and Polymers Using Monodisperse Poly(2,3-dihydroxypropyl Methacrylate-co-Ethylene Dimethacrylate) Beads via Normal-Phase and Hydrophilic-Interaction HPLC

Microparticulate, monosized, and macroporous poly(2,3-dihydroxypropyl methacrylate- co -ethylene dimethacrylate) beads have been used as a stationary phase for HPLC separations of hydrophilic oligomers and polymers. Homogeneous coverage of the sorbent surface with a large number of chemically equivalent diol functionalities affords suitable retentivity in both normal-phase and hydrophilic-interaction chromatographic modes and enables the separations of water-soluble oligomers and polymers. Chromatographic properties of this stationary phase are demonstrated on a variety of separations of poly(oxyalkylene)s, polyvinylpyrrolidones, and polysaccharides.