Response to Comment on “RNA-guided DNA insertion with CRISPR-associated transposases”
Letter 2020 en
Authors
JS
Jonathan Strecker
AL
Alim Ladha
KM
Kira S. Makarova
Abstract
1 min read
Rice et al . suggest that the CRISPR-associated transposase ShCAST system could lead to additional insertion products beyond simple integration of the donor. We clarify the outcomes of ShCAST-mediated insertions in Escherichia coli , which consist of both simple insertions and integration of the donor plasmid. This latter outcome can be avoided by use of a 5′ nicked DNA donor.
Felix Nicolaus, Ane Metola, Daphne Mermans, Amanda Liljenström, Ajda Krč, Salmo Mohammed Abdullahi, Matthew Zimmer, Thomas F. Miller, Gunnar Von Heijne
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