Primary Structure of Cytochrome <i>b</i>₅ from Cauliflower (<i>Brassica oleracea</i> L.) Deduced from Peptide and cDNA Sequences

Cytochrome b(5) is a microsomal protein that functions as an intermediate electron donor in fatty acid desaturation and other oxidation/reduction reactions. cDNA clones were isolated from cauliflower (Brassica oleracea L.) by using oligonucleotides based on the partial amino acid sequence of the protein. The deduced amino acid sequence of the polypeptide exhibited approximately 30% sequence identity with the homologous protein from vertebrates.