Optimization of chitosan-based composite and bi-layered scaffolds produced by particles aggregation for osteochondral tissue engineering: Influence of hydroxylapatite

[Excerpt] Osteochondral tissue engineering presents a challenge to the present research due to requirements’ combination of both bone and cartilage tissue engineering. In the present study, bilayered chitosan scaffolds are proposed based in the optimization of polymeric and composite scaffolds. µ-CT was carried out for accurate morphometric characterization quantifying porosity, interconnectivity, ceramic content, particles and pores size. The results showed that the developed scaffolds are highly interconnected and present ideal pore size range, being morphometrically adequate for the proposed applications. [...]