One-step light-up metabolic probes for <i>in situ</i> discrimination and killing of intracellular bacteria

Eric Y. Yu; Michelle M. S. Lee; Joe H. C. Chau; Kristy W. K. Lam; Hojeong Park; Ryan T. K. Kwok; Jacky W. Y. Lam; Yuanyuan Li; Ben Zhong Tang

doi:10.1039/d1qm01495a

Abstract

1 min read

TPEPy-Ala and TPAPy-Kdo with metabolic moieties can be directly incorporated into the bacterial cell envelopes and light up intracellular bacteria. Additionally, the metabolic probes can effectively eliminate labeled bacteria in situ with minimal host cell cytotoxicity via photodyanmic therapy.

Related publications

Article2025

Photosensitizing Quantum Dot Killers Encoded by Bivalent DNA for Sequential Cell Penetration, Intracellular Bacterial Imaging, and Targeted Elimination

Ling‐Hong Xiong, Pengzhi Hu, Jing Zhang, Jie Sun, Jiangtao Geng, Ming‐Peng Zhuo, Ben Zhong Tang, Xuewen He

Preprint2020

Early invasion of uropathogenic<i>Escherichia coli</i>into the bladder wall by solitary bacteria that are protected from antibiotics and neutrophil swarms in an organoid model

Kunal Sharma, Vivek V. Thacker, Neeraj Dhar, François Signorino‐Gelo, Maria Clapés Cabrer, A. Dubois, Jasper Mullenders, Graham Knott, Hans Clevers, John D. McKinney

Article1999

Modulation of Endocytosis in Nuclear Factor IL-6(−/−) Macrophages Is Responsible for a High Susceptibility to Intracellular Bacterial Infection

Javier Pizarro‐Cerdá, Michel Desjardins, Edgardo Moreno, Akira Shizuo, Jean‐Pierre Gorvel

The Journal of Immunology

Article2016

Antibacterial Surface Design of Titanium-Based Biomaterials for Enhanced Bacteria-Killing and Cell-Assisting Functions Against Periprosthetic Joint Infection

Jiaxing Wang, Jinhua Li, Qian Shi, Geyong Guo, Qiaojie Wang, Jin Tang, Hao Shen, Xuanyong Liu, Xianlong Zhang, Paul Kim Ho Chu

Preprint2020

Selection for reducing energy cost of protein production drives the GC content and amino acid composition bias in gene transfer agents

Roman Kogay, Yuri I. Wolf, Eugene V Koonin, Olga Zhaxybayeva