Monitoring Osteoblastic Differentiation with Multivariate Analysis of Fluorescence Lifetime Imaging

Carlo Alonzo; Kyle P. Quinn; Rebecca S. Hayden; David Kaplan; Irene Georgakoudi

doi:10.1364/omp.2013.mth1c.7

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Monitoring Osteoblastic Differentiation with Multivariate Analysis of Fluorescence Lifetime Imaging

Article 2013 en

Abstract

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Osteoblastic differentiation of mesenchymal stem cells was monitored via time-resolved fluorescence of endogenous NADH. Multivariate analysis of fluorescence decay parameters yielded canonical variables that track cell differentiation status.

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