Lipid Peroxidation and Antioxidant System in Erythrocytes of Brucella Vaccinated and Challenged Goats

The immune responses and protection in Brucella vaccination are mediated through interaction of Reactive oxygen species, free radicals and antioxidant system.These can be assessed by various oxidative stress and antioxidant biomarkers by measuring rate of lipid peroxidation (LPO) and activity of antioxidant enzymes in erythrocytes.In the present study interaction of Reactive oxygen species, free radicals and antioxidant system was evaluated during Rev.1 vaccination and challenge with virulent Brucella melitensis biovar 3 in adult healthy Brucella free pure bred Jamunapari goats.Vaccinated goats were challenged on 28 th day post vaccination.The level of Catalase, GST and MDA formation increased significantly whereas level of SOD and GSH reduced significantly after vaccination.The challenge with virulent culture on 28 th post vaccination initially reduced the blood erythrocyte level of Catalase, GST, GSH and MDA formation on 14 th day with subsequent increase on 28 th day post challenge excluding SOD and GSH.The erythrocyte level of catalase, GST and MDA formation reduced on 60 th day post vaccination.Based on mechanism involved in ROS production and antioxidant mechanism the levels of CAT and GST acted in synergism to immunization with Rev.1 by maintaining ROS in goat erythrocytes.As antioxidant system activation is required to obtain protection against Brucella, thus CAT and GST can be used as oxidative stress and antioxidant fingerprint markers of redox balance in brucellosis and its vaccination.