Label-free multiphoton metabolic imaging of a 3D human brain-like tissue model of Alzheimer's disease

We aim to highlight the potential of label-free, TPEF metabolic imaging to gain unique insights in the development of Alzheimer’s disease (AD). Using an engineered brain tissue model infected with HSV-1 to induce AD phenotypes, we focus on dynamic changes associated with metabolic function, as reported by both the redox ratio and mitochondrial clustering, and the development of Aβ+ fibrillar plaque like formations (PLFs).