Inverse colloidal crystal membranes for hydrophobic interaction membrane chromatography

J. Sep. Sci. 2015, 38, 3262-3270 DOI: 10.1002/jssc.201500551 This cover picture illustrates an inter-laboratory study on the robustness and portability of capillary electrophoresis-mass spectrometry that is presented in this issue. Thirteen independent laboratories located around the globe as indicated on the map were participating in this study. The sheath-flow capillary electrophoresis-mass spectrometry interface used by most participating groups is depicted schematically on the right hand side. Migration time, peak height and peak area of 10 representative target peptides of a trypsin-digested protein sample were determined by every laboratory. The figures in the middle and left hand side show the final results obtained for means, repeatability (precision within a laboratory) and reproducibility (precision between laboratories). This study demonstrates that capillary electrophoresis-mass spectrometry is robust enough to allow a method transfer across multiple laboratories and should promote a more widespread use of this technology in biopharmaceutical analysis and related fields.