Integrity of Cell-bound Poly(I).Poly(C)

SUMMARY The acid-insoluble radioactivity associated with the cells following incubation of the cells with [3H]-labelled poly(I).poly(C) [poly(I).poly(C)* for 1 h has been analysed by sucrose gradient velocity ultracentrifuging. Intact (poly(I).poly(C)* was recovered from primary rabbit kidney (PRK) cells, partially degraded poly(I). poly(C)* was recovered from mouse L 929 cells and completely degraded material was recovered from HeLa and VERO cells. Priming of the cells with homologous interferon did not alter the sedimentation profile of cell-associated poly(I).poly(C)* in PRK, L 929 or HeLa cells.