In Vivo Engraftment Potential of human Bone Marrow and Amniotic Fluid Stem Cells Cultured under Osteogenic Conditions

The potential immune privilege presented by mesenchymal stem cells (MSCs) makes them a promising population in tissue engi- neering and regenerative medicine. In this study, we investigated the effect of culture medium (expansion versus osteogenic media) over the in vivo potential of adult stem cells derived from human bone marrow (hBMMSCs) and amniotic fluid (hAFSCs).To this end, we isolated hAFSCs from day 6 supernatant of the cultures of amniotic fluid obtained from amniocentesis, and hBMMSCs were a kind gift from Biopredic. Cells were maintained in culture until confluence either in expansion or in osteogenic media (7 days), and then transplanted into 58 to 62 day-old foetal sheep at a concentration of 1