Histopathology and Immunohistochemistry v1

Histopathology and immunohistochemistry (IHC) were performed on pancreatic tissue sections and tissue microarrays (TMAs). Tissues were fixed, embedded in paraffin, and sectioned for staining with hematoxylin and eosin (H&E). For IHC, sections were incubated with primary antibodies, followed by secondary HRP-conjugated antibodies and visualization with diaminobenzidine (DAB). Images were captured using light and fluorescent microscopy. TMA slides were analyzed for protein expression levels, and results were scored based on staining intensity and extent.