Abstract
1 min readMetagenomics deal with analysis of genetic material from environmental samples. The ultimate goal is to reconstruct entire genomes of unknown microbial species. We present a sample preparation method using the Fluidigm© C1 microfluidic platform that integrates throughput of shotgun sequencing with bioinformatics simplicity of single cell microfluidics. Our method is tested with a benchmark sample and characterize its mapping quality and coverage uniformity. We conclude that our microfluidic based pipeline is suitable for metagenomic applications.
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